YOYO-3
Based on 1 Customer Validation
YOYO-3 is a trimethine cyanine homodimer dye. YOYO-3 intercalates into AF488-tagged dsDNA molecules at the single-molecule level. YOYO-3 has a greater affinity for DNA than do EB and other Cyanine dyes.
For research use only. We do not sell to patients.
- Purity: 98.68%
- CAS No.: 156312-20-8
- Formula: C53H62I4N6O2
- Molecular Weight:1322.72
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
YOYO-3 (0.10-0.80 μM; 10 min) binds to dsDNA as a single intercalator[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 156312-20-8
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Appearance Solid
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Molecular Weight 1322.72
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Formula C53H62I4N6O2
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Color Dark purple to black
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SMILES
C[N+](C)(CCC[N+](C)(CCC[N+]1=CC=C(C2=C1C=CC=C2)/C=C/C=C3OC4=C(N/3C)C=CC=C4)C)CCC[N+](C5=C6C=CC=C5)=CC=C6/C=C/C=C7N(C)C8=C(C=CC=C8)O\7.[I-].[I-].[I-].[I-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (75.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7560 mL | 3.7801 mL | 7.5602 mL | 18.9004 mL |
| 5 mM | 0.1512 mL | 0.7560 mL | 1.5120 mL | 3.7801 mL | |
| 10 mM | 0.0756 mL | 0.3780 mL | 0.7560 mL | 1.8900 mL | |
| 15 mM | 0.0504 mL | 0.2520 mL | 0.5040 mL | 1.2600 mL | |
| 20 mM | 0.0378 mL | 0.1890 mL | 0.3780 mL | 0.9450 mL | |
| 25 mM | 0.0302 mL | 0.1512 mL | 0.3024 mL | 0.7560 mL | |
| 30 mM | 0.0252 mL | 0.1260 mL | 0.2520 mL | 0.6300 mL | |
| 40 mM | 0.0189 mL | 0.0945 mL | 0.1890 mL | 0.4725 mL | |
| 50 mM | 0.0151 mL | 0.0756 mL | 0.1512 mL | 0.3780 mL | |
| 60 mM | 0.0126 mL | 0.0630 mL | 0.1260 mL | 0.3150 mL |