β2AR agonist 6

β2AR agonist 6 (compound 150) (0.005-10000 nM; incubated with hM₃-CHO cell membranes; single treatment; 2 or 24 h) exhibits sustained high-affinity binding to hM₃^[1].
β2AR agonist 6 (0.5-10000 nM; treatment of CHRM3-CHO cells; single pre-incubation; 0.25 or 1 h) antagonizes acetylcholine-induced calcium signals^[1].
β2AR agonist 6 (0.005-10000 nM; tested in [³H]-NMS competitive binding assay using CHO cell membranes expressing hM₃; single treatment; 2 or 24 h) binds to hM₃, with K_i values of 1.89 nM and 1.95 nM after 2 h and 24 h of incubation, respectively^[1].

β2AR agonist 6 (0.5-10000 nM; tested via FLIPR calcium flux assay using CHO cells expressing hM3; single pre-incubation; 0.25 or 1 h) antagonizes acetylcholine-induced hM3 signaling, with KB values of 64.9 nM and 66.1 nM, respectively^[1].
β2AR agonist 6 (0.5 pM-1 μM; β2 receptor cAMP/HTRF assay; single treatment; detection performed 1 h after 1 h of stimulation) activates hβ2 with an EC50 of 0.266 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.