Coumarin 6H
Based on 1 Customer Validation
Coumarin 6H, a Coumarin (HY-N0709) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions.
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- 純度: 99.05%
- CAS 番号: 58336-35-9
- 分子式: C15H15NO2
- 分子量:241.29
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
生物活性
The Coumarin 6H with twistblocked and stronger electron-donating julolidine group exhibits higher quantum yield, better biocompatibility, as well as less background fluorescence when suppressed by a fluorescence quenching group. Thus, the Coumarin 6H in probe LH-1 may help to reduce the background fluorescence. In probe LH-1, the fluorescence of Coumarin 6H fluorophore not only can be quenched by the rotation of N-N group, but also can be quenched by the 2,4-dinitrobenzenze group via donor-excited photoinduced electron transfer (d-PET) mechanism[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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化学情報
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CAS 番号 58336-35-9
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性状 Solid
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分子量 241.29
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分子式 C15H15NO2
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Color Yellow to brown
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SMILES
O=C1C=CC2=CC3=C4N(CCCC4=C2O1)CCC3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 12.5 mg/mL (51.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (274 KB)
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SDS (393 KB)
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- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. P M French, et al. Passively mode-locked cw Coumarin 6 ring dye laser. Opt Lett. 1989 Feb 15;14(4):217-8. [Content Brief]
[2]. Zhixiang Han, et al. A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish. Anal Biochem. 2020 Aug 1;602:113795. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1444 mL | 20.7220 mL | 41.4439 mL | 103.6098 mL |
| 5 mM | 0.8289 mL | 4.1444 mL | 8.2888 mL | 20.7220 mL | |
| 10 mM | 0.4144 mL | 2.0722 mL | 4.1444 mL | 10.3610 mL | |
| 15 mM | 0.2763 mL | 1.3815 mL | 2.7629 mL | 6.9073 mL | |
| 20 mM | 0.2072 mL | 1.0361 mL | 2.0722 mL | 5.1805 mL | |
| 25 mM | 0.1658 mL | 0.8289 mL | 1.6578 mL | 4.1444 mL | |
| 30 mM | 0.1381 mL | 0.6907 mL | 1.3815 mL | 3.4537 mL | |
| 40 mM | 0.1036 mL | 0.5180 mL | 1.0361 mL | 2.5902 mL | |
| 50 mM | 0.0829 mL | 0.4144 mL | 0.8289 mL | 2.0722 mL |