Fast Red Violet LB
Fast Red Violet LB is a dye for staining tartrate resistant acid phosphatase (TRAP). Fast Red Violet LB can be used for alkaline phosphatase (ALP) activity staining.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 32348-81-5
- 分子式: C14H11Cl2N3O
- 分子量:308.16
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution[2]
1.1 Prepare 0.1% Fast Red Violet LB solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 32348-81-5
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分子量 308.16
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分子式 C14H11Cl2N3O
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SMILES
N#[N+]C1=CC(Cl)=C(NC(C2=CC=CC=C2)=O)C=C1C.[Cl-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Yan D, et al. Genetic background influences fluoride's effects on osteoclastogenesis. Bone. 2007 Dec;41(6):1036-44. [Content Brief]
[2]. Kotobuki N, et al. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs. 2004 Jan;28(1):33-9. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Fast Red Violet LB 関連分類
HY-D1491 関連分類
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