Fast Red Violet LB Zinc chloride

Name: Fast Red Violet LB Zinc chloride
Brand: MedChemExpress
SKU: HY-D1491A
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1491A Pureté: 99.50%: Fiche technique
COA

SDS
Instruction de manipulation
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Fast Red Violet LB Zinc chloride is a stain that stains tartrate-resistant acid phosphatase (TRAP) and Fast Red Violet LB Zinc chloride can be used to stain alkaline phosphatase (ALP) activity.

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Fast Red Violet LB Zinc chloride Chemical Structure

Size	Stock	Quantité
Échantillon gratuit (0.1 - 0.2 mg)	Appliquer maintenant
Échantillon gratuit (0.1 - 0.2 mg)	Appliquer maintenant
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
250 mg	En stock	Estimated Time of Arrival: December 31
500 mg	Obtenir un devis
1 g	Obtenir un devis

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Based on 1 publication(s) in Google Scholar

Other Forms of Fast Red Violet LB Zinc chloride:

Fast Red Violet LB Obtenir un devis

Recommandations en vedette

•Related Small Molecules:

•Related Proteins:

Activité biologique
Pureté et documentation
Références
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Description

Fast Red Violet LB Zinc chloride is a stain that stains tartrate-resistant acid phosphatase (TRAP) and Fast Red Violet LB Zinc chloride can be used to stain alkaline phosphatase (ALP) activity^[1]^[2].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution^[2]
1.1 Prepare 0.1% Fast Red Violet LB chloride solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

340.86

Formule

C₁₄H₁₁ClN₃O.1/2ZnCl₂

Appearance

Solid

Color

Off-white to yellow

SMILES

N#[N+]C1=CC(Cl)=C(NC(C2=CC=CC=C2)=O)C=C1C.Cl[Zn]Cl.[1/2]

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 66.67 mg/mL (195.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9338 mL	14.6688 mL	29.3376 mL
5 mM	0.5868 mL	2.9338 mL	5.8675 mL
10 mM	0.2934 mL	1.4669 mL	2.9338 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Pureté et documentation

Purity: 99.50%

Select Batch:

Fiche technique (275 KB) SDS (251 KB)

COA (252 KB) HNMR (273 KB) RP-HPLC (233 KB) MS (73 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Yan D, et al. Genetic background influences fluoride's effects on osteoclastogenesis. Bone. 2007 Dec;41(6):1036-44. [Content Brief]

[2]. Kotobuki N, et al. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs. 2004 Jan;28(1):33-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9338 mL	14.6688 mL	29.3376 mL	73.3439 mL
	5 mM	0.5868 mL	2.9338 mL	5.8675 mL	14.6688 mL
	10 mM	0.2934 mL	1.4669 mL	2.9338 mL	7.3344 mL
	15 mM	0.1956 mL	0.9779 mL	1.9558 mL	4.8896 mL
	20 mM	0.1467 mL	0.7334 mL	1.4669 mL	3.6672 mL
	25 mM	0.1174 mL	0.5868 mL	1.1735 mL	2.9338 mL
	30 mM	0.0978 mL	0.4890 mL	0.9779 mL	2.4448 mL
	40 mM	0.0733 mL	0.3667 mL	0.7334 mL	1.8336 mL
	50 mM	0.0587 mL	0.2934 mL	0.5868 mL	1.4669 mL
	60 mM	0.0489 mL	0.2445 mL	0.4890 mL	1.2224 mL
	80 mM	0.0367 mL	0.1834 mL	0.3667 mL	0.9168 mL
	100 mM	0.0293 mL	0.1467 mL	0.2934 mL	0.7334 mL