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  3. Fast Red Violet LB Zinc chloride

Fast Red Violet LB Zinc chloride is a stain that stains tartrate-resistant acid phosphatase (TRAP) and Fast Red Violet LB Zinc chloride can be used to stain alkaline phosphatase (ALP) activity.

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Fast Red Violet LB Zinc chloride

Fast Red Violet LB Zinc chloride 화학구조

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
250 mg 해외재고보유
500 mg   견적 받기  
1 g   견적 받기  

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고객리뷰

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Other Forms of Fast Red Violet LB Zinc chloride:

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  • 순도&문서

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제품 설명

Fast Red Violet LB Zinc chloride is a stain that stains tartrate-resistant acid phosphatase (TRAP) and Fast Red Violet LB Zinc chloride can be used to stain alkaline phosphatase (ALP) activity[1][2].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution[2]
1.1 Prepare 0.1% Fast Red Violet LB chloride solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

340.86

화학식

C14H11ClN3O.1/2ZnCl2

Appearance

Solid

Color

Off-white to yellow

SMILES

N#[N+]C1=CC(Cl)=C(NC(C2=CC=CC=C2)=O)C=C1C.Cl[Zn]Cl.[1/2]

선적

Room temperature in continental US; may vary elsewhere.

보관
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 66.67 mg/mL (195.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9338 mL 14.6688 mL 29.3376 mL
5 mM 0.5868 mL 2.9338 mL 5.8675 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서

Purity: 99.50%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9338 mL 14.6688 mL 29.3376 mL 73.3439 mL
5 mM 0.5868 mL 2.9338 mL 5.8675 mL 14.6688 mL
10 mM 0.2934 mL 1.4669 mL 2.9338 mL 7.3344 mL
15 mM 0.1956 mL 0.9779 mL 1.9558 mL 4.8896 mL
20 mM 0.1467 mL 0.7334 mL 1.4669 mL 3.6672 mL
25 mM 0.1174 mL 0.5868 mL 1.1735 mL 2.9338 mL
30 mM 0.0978 mL 0.4890 mL 0.9779 mL 2.4448 mL
40 mM 0.0733 mL 0.3667 mL 0.7334 mL 1.8336 mL
50 mM 0.0587 mL 0.2934 mL 0.5868 mL 1.4669 mL
60 mM 0.0489 mL 0.2445 mL 0.4890 mL 1.2224 mL
80 mM 0.0367 mL 0.1834 mL 0.3667 mL 0.9168 mL
100 mM 0.0293 mL 0.1467 mL 0.2934 mL 0.7334 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Fast Red Violet LB Zinc chloride
Cat. No.:
HY-D1491A
수량:
MCE Japan Authorized Agent: