Fast Red Violet LB Zinc chloride
Based on 1 Customer Validation
Fast Red Violet LB Zinc chloride is a stain that stains tartrate-resistant acid phosphatase (TRAP) and Fast Red Violet LB Zinc chloride can be used to stain alkaline phosphatase (ALP) activity.
For research use only. We do not sell to patients.
- Purity: 99.50%
- Formula: C14H11ClN3O.1/2ZnCl2
- Molecular Weight:340.86
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Working Solution[2]
1.1 Prepare 0.1% Fast Red Violet LB chloride solution.
2. Specific Staining Steps
2.1 Wash cells with PBS to remove residual reagents.
2.2 Fix cells with 4% paraformaldehyde for 10 min.
2.3 Wash with PBS to remove paraformaldehyde.
2.4 Incubate cells with Fast Red Violet LB working solution and 0.1% naphthol AS-MX phosphate at room temperature for 10 min.
2.5 Wash cells thoroughly with PBS to remove unbound dye.
2.6 Observe alkaline phosphatase (ALP)-positive cells under a microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 340.86
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Formula C14H11ClN3O.1/2ZnCl2
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Color Off-white to yellow
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SMILES
N#[N+]C1=CC(Cl)=C(NC(C2=CC=CC=C2)=O)C=C1C.Cl[Zn]Cl.[1/2]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 66.67 mg/mL (195.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Yan D, et al. Genetic background influences fluoride's effects on osteoclastogenesis. Bone. 2007 Dec;41(6):1036-44. [Content Brief]
[2]. Kotobuki N, et al. Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs. 2004 Jan;28(1):33-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9338 mL | 14.6688 mL | 29.3376 mL | 73.3439 mL |
| 5 mM | 0.5868 mL | 2.9338 mL | 5.8675 mL | 14.6688 mL | |
| 10 mM | 0.2934 mL | 1.4669 mL | 2.9338 mL | 7.3344 mL | |
| 15 mM | 0.1956 mL | 0.9779 mL | 1.9558 mL | 4.8896 mL | |
| 20 mM | 0.1467 mL | 0.7334 mL | 1.4669 mL | 3.6672 mL | |
| 25 mM | 0.1174 mL | 0.5868 mL | 1.1735 mL | 2.9338 mL | |
| 30 mM | 0.0978 mL | 0.4890 mL | 0.9779 mL | 2.4448 mL | |
| 40 mM | 0.0733 mL | 0.3667 mL | 0.7334 mL | 1.8336 mL | |
| 50 mM | 0.0587 mL | 0.2934 mL | 0.5868 mL | 1.4669 mL | |
| 60 mM | 0.0489 mL | 0.2445 mL | 0.4890 mL | 1.2224 mL | |
| 80 mM | 0.0367 mL | 0.1834 mL | 0.3667 mL | 0.9168 mL | |
| 100 mM | 0.0293 mL | 0.1467 mL | 0.2934 mL | 0.7334 mL |
Fast Red Violet LB Zinc chloride Related Classifications
HY-D1491A Related Classifications
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