Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots

  • J Ginseng Res. 2014 Apr;38(2):116-22. doi: 10.1016/j.jgr.2013.10.002.
Jin-Gyeong Cho  1 ,  Seo-Ji In  1 ,  Ye-Jin Jung  1 ,  Byeong-Ju Cha  1 ,  Dae-Young Lee  2 ,  Yong-Bum Kim  3 ,  Myeonghun Yeom  4 ,  Nam-In Baek  1
Affiliations
  • 1. Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
  • 2. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.
  • 3. Technology Services Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Korea.
  • 4. Amorepacific Corporation Research and Development Center, Yongin, Korea.
PMID: 24748835 DOI: 10.1016/j.jgr.2013.10.002
Abstract

Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as (1)H-(1)H correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published (1)H- and (13)C-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.

Keywords
HPLC; NMR; Panax ginseng; fast atom bombardment/MS; triol ginsenoside.
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