Thymol Blue sodium
Based on 1 Customer Validation
Thymol Blue sodium is an acid-base indicator used to indicate changes in pH. Thymol Blue sodium fades from red to yellow at pH 1.2 to 2.8 and from yellow to blue at pH 8.0 to 9.6. Quantitative detection is achieved through the absorption peak shift (435 nm/596 nm) of the UV-visible spectrum. Thymol blue sodium can be fixed in a silica gel matrix through sol-gel technology to form a solid-state sensor for in-situ pH measurement in the marine environment and acid-base monitoring in the biomedical field.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 62625-21-2
- 分子式: C27H29NaO5S
- 分子量:488.57
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保管条件:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
生物活性
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Thymol Blue acid-base indicator protocol (solution state)[1]:
1. Solution preparation
Dissolve Thymol Blue sodium salt in ethanol (or water/ethanol mixed solvent) to prepare a 0.1% (w/v) stock solution.
2. pH response test
Take the solution to be tested and add 1-2 drops of Thymol Blue indicator:
Acidic conditions (pH<1.7) The solution turns red (HI- form).
Neutral to weakly alkaline (1.7≤pH≤8.9: the solution is yellow (intermediate state.
Strongly alkaline (pH>8.9): the solution is blue (I2- form).
3. Quantitative detection
Use a spectrophotometer to measure the absorbance at 435 nm (HI-) and 596 nm (I2-), calculate the ratio R=A596/A435, and calculate the pH value using the ionization formula.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 62625-21-2
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性状 Solid
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分子量 488.57
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分子式 C27H29NaO5S
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Color Yellow to brown
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SMILES
[Na].O=S1(=O)OC(C2=CC(=C(O)C=C2C)C(C)C)(C3=CC(=C(O)C=C3C)C(C)C)C=4C=CC=CC41
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
溶剤 & 溶解度
DMSO : 6.72 mg/mL (13.75 mM; ultrasonic and adjust pH to 4 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (282 KB)
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SDS (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0468 mL | 10.2339 mL | 20.4679 mL | 51.1697 mL |
| 5 mM | 0.4094 mL | 2.0468 mL | 4.0936 mL | 10.2339 mL | |
| 10 mM | 0.2047 mL | 1.0234 mL | 2.0468 mL | 5.1170 mL |