Bay K 8644
Based on 8 publication(s) in Google Scholar
Bay K 8644 ((±)-Bay K 8644) is a racemate consisting of two isomers (R)-(+)-Bay-K-8644 and (S)-(-)-Bay-K-8644. Bay K 8644 is a L-type Ca2+ channel agonist with an EC50 of 17.3 nM. Bay K 8644 increases Ca2+ influx through sarcolemmal Ca2+ channels by increasing the open time of the channel. Bay K 8644 has vasoconstrictive effects.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.96%
- CAS No.: 71145-03-4
- 화학식: C16H15F3N2O4
- 분자량:356.30
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) Bay K 8644
More- Int J Biol Macromol. 2025 Apr 26:143548. [Abstract]
- Phytomedicine. 2024 Jan:123:155213. [Abstract]
- Bioorg Chem. 2026 Mar:170:109509. [Abstract]
- Neuroscience. 2022 Jun 1;492:47-57. [Abstract]
- Fitoterapia. 2026 Mar 17.
- Fitoterapia. 2025 Oct 6:187:106924. [Abstract]
- Cells Dev. 2026 Mar:185:204073. [Abstract]
- University of Groningen. 2025 Jul 4.
Customer Validation & Images
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In Vivo Efficacy Study
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RT-PCR
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Calcium Channel Isoforms
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Biological Activity
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L-type calcium channel 17.3 nM (EC50) |
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Cell Line
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Type | Value | Description | References |
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| CHO | EC50 |
32.7 μM
Compound: 57
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Agonist activity at mouse TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium influx
Agonist activity at mouse TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium influx
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[PMID: 20356305] |
| GH3 | EC50 |
0.015 μM
Compound: BAY-K-8644
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Tested for 45 [Ca2+] uptake in rat pituitary growth hormone3 (GH3) cells
Tested for 45 [Ca2+] uptake in rat pituitary growth hormone3 (GH3) cells
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[PMID: 7692047] |
In newborn rat ventricular cardiomyocytes, Bay K 8644 (1 μM) treatment increases L-type calcium current density in 2-day-old cells. The higher increase of L-type calcium current density by Bay K 8644 in 2-day- than in 7-day-old cultured cells could be interpreted as the result of a difference in the phosphorylation level of calcium channels for each stage of development[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 71145-03-4
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Appearance Solid
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분자량 356.30
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화학식 C16H15F3N2O4
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Color Light yellow to yellow
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SMILES
O=C(C1=C(C)NC(C)=C([N+]([O-])=O)C1C2=C(C(F)(F)F)C=CC=C2)OC
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Synonyms
(±)-Bay K 8644
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Genome-wide identification and expression analysis of neuropeptide G protein-coupled receptors in Urechis unicinctus (Annelida, Echiura). [Abstract]2025 Apr 26:143548. PMID: 40294673
Bay K 8644 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Apr 26:143548. [Abstract]
Bay K 8644 (3 μM; 48 h). Settlement rate of the larvae treated with calmidazolium and Bay K 8644.
Bay K 8644 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Apr 26:143548. [Abstract]
Bay K 8644 (3 μM; 48 h). The relative expressions of CALM in the larvae treated with Bay K 8644 of cAMP and Ca2+ pathway.
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Phytomedicine
Unraveling the anti-primary dysmenorrhea mechanism of Ainsliaea fragrans Champ. extract by the integrative approach of network pharmacology and experimental verification. [Abstract]2024 Jan:123:155213. PMID: 37980805
Bay K 8644 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jan:123:155213. [Abstract]
Representative physiographic recording showing AF-ext (0.25 ~ 1 mg/ml) on mice uterine strips induced by l-type Ca2+ channel agonist Bay K 8644 (10 μM).
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Bioorg Chem
Lometrexol targets MRPL2 to suppress NSCLC via dual regulation of mitochondrial ribosomal activity and nuclear PDCD11/ Ca2+ signaling. [Abstract]2026 Mar:170:109509. PMID: 41558196 -
Neuroscience
TGF-β1 Protects Trauma-injured Murine Cortical Neurons by Upregulating L-type Calcium Channel Cav1.2 via the p38 Pathway. [Abstract]2022 Jun 1;492:47-57. PMID: 35460836 -
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Fitoterapia
Integrated network pharmacology and therapeutic verification of Dendrobium officinale flower on primary dysmenorrhea via inhibition of the TLR4/NF-κB/COX-2 pathway. [Abstract]2025 Oct 6:187:106924. PMID: 41061996
Bay K 8644 purchased from MedChemExpress. Usage Cited in: Fitoterapia. 2025 Oct 6:187:106924. [Abstract]
Representative physiographic recording of uterus contractions inhibited by DOF-EA (0.25–1 mg/mL) on mice uterine strips induced by Ca2+ channel agonist Bay K 8644 (20 μM), with inhibition rate shown in the bar chart.
Bay K 8644 purchased from MedChemExpress. Usage Cited in: Fitoterapia. 2025 Oct 6:187:106924. [Abstract]
Representative physiographic recording of uterus contractions inhibited by DOF-EA (0.25–1 mg/mL) on mice uterine strips induced by Ca2+ channel agonist Bay K 8644 (20 μM), with inhibition rate shown in the bar chart.
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Cells Dev
Small molecules induces dorsal root ganglion satellite glial cells to differentiate into sensory neuron-like cells. [Abstract]2026 Mar:185:204073. PMID: 41690597 -
용액&용해도
DMSO : 83.33 mg/mL (233.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.84 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.69 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. W Schreibmayer, et al. Kinetic modulation of guinea-pig cardiac L-type calcium channels by fendiline and reversal of the effects of Bay K 8644. Br J Pharmacol. 1992 May;106(1):151-6. [Content Brief]
[2]. G A Rae, et al. Interactions of calcium antagonists and the calcium channel agonist Bay K 8644 on neurotransmission of the mouse isolated vas deferens. Br J Pharmacol. 1989 Feb;96(2):333-40. [Content Brief]
[3]. H Satoh, et al. Bay K 8644 increases resting Ca2+ spark frequency in ferret ventricular myocytes independent of Ca influx: contrast with caffeine and ryanodine effects. Circ Res. 1998 Dec 14-28;83(12):1192-204. [Content Brief]
[4]. J P Gomez, et al. Effects of Bay K 8644 on L-type calcium current from newborn rat cardiomyocytes in primary culture. J Mol Cell Cardiol. 1996 Oct;28(10):2217-29. [Content Brief]
[5]. N Ives,et al. BAY k 8644, a calcium channel agonist, reverses hypotension in endotoxin-shocked rats. Eur J Pharmacol. 1986 Nov 4;130(3):169-75. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8066 mL | 14.0331 mL | 28.0662 mL | 70.1656 mL |
| 5 mM | 0.5613 mL | 2.8066 mL | 5.6132 mL | 14.0331 mL | |
| 10 mM | 0.2807 mL | 1.4033 mL | 2.8066 mL | 7.0166 mL | |
| 15 mM | 0.1871 mL | 0.9355 mL | 1.8711 mL | 4.6777 mL | |
| 20 mM | 0.1403 mL | 0.7017 mL | 1.4033 mL | 3.5083 mL | |
| 25 mM | 0.1123 mL | 0.5613 mL | 1.1226 mL | 2.8066 mL | |
| 30 mM | 0.0936 mL | 0.4678 mL | 0.9355 mL | 2.3389 mL | |
| 40 mM | 0.0702 mL | 0.3508 mL | 0.7017 mL | 1.7541 mL | |
| 50 mM | 0.0561 mL | 0.2807 mL | 0.5613 mL | 1.4033 mL | |
| 60 mM | 0.0468 mL | 0.2339 mL | 0.4678 mL | 1.1694 mL | |
| 80 mM | 0.0351 mL | 0.1754 mL | 0.3508 mL | 0.8771 mL | |
| 100 mM | 0.0281 mL | 0.1403 mL | 0.2807 mL | 0.7017 mL |
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