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Cat. No.: HY-D3389

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sCy5DL-amide

sCy5DL-amide is an amidated fluorescent D-amino acid conjugated to Sulfo-Cyanine 5, and a peptidoglycan labeling agent. sCy5DL-amide incorporates into bacterial peptidoglycan cell wall via transpeptidase-mediated reactions, replacing the fourth or fifth D-amino acid of the peptidoglycan stem peptide for visualization of nascent peptidoglycan biosynthesis. sCy5DL-amide produces clear cell outline, septum labeling, and high localization density in Bacillus subtilis, including a 'V-shape' pattern at cell-cell contact areas. sCy5DL-amide exhibits robust incorporation into Gram-positive bacteria and mycobacteria, with reduced incorporation into Gram-negative bacteria (Ex/Em = 646/666 nm).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

화학식: C₃₈H₅₁N₅O₈S₂
분자량:769.97

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

sCy5DL-amide Chemical Structure

사이즈

가격

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수량

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

In Vitro

sCy5DL-amide (0.5 mM; 20 min) efficiently incorporates into the cell wall of Bacillus subtilis, enabling clear visualization of cell outlines and septa via widefield fluorescence imaging^[1].
sCy5DL-amide (1 mM; 1-2 cell cycle duration) efficiently incorporates into the cell walls of Mycobacterium marinum and Mycobacterium tuberculosis, enabling high-resolution visualization of cell wall ultrastructure via SMLM imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

분자량 769.97
화학식 C₃₈H₅₁N₅O₈S₂
SMILES

CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)N(CCCCCC(NCCCC[C@H](C(N)=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Zhang C, et al. Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids. Methods Mol Biol. 2024;2727:83-94. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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