2. Academic Validation
  3. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry

CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry

  • Science. 2006 May 26;312(5777):1220-3. doi: 10.1126/science.1127883.
M Vig 1,  C Peinelt,  A Beck,  D L Koomoa,  D Rabah,  M Koblan-Huberson,  S Kraft,  H Turner,  A Fleig,  R Penner,  J-P Kinet
Affiliations

Affiliation

  • 1 Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA. [email protected]
PMID: 16645049 DOI: 10.1126/science.1127883
Abstract

Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC Channel itself, a subunit of it, or a component of the CRAC signaling machinery.

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