2. Academic Validation
  3. FERM domain-containing protein FRMD5 regulates cell motility via binding to integrin β5 subunit and ROCK1

FERM domain-containing protein FRMD5 regulates cell motility via binding to integrin β5 subunit and ROCK1

  • FEBS Lett. 2014 Nov 28;588(23):4348-56. doi: 10.1016/j.febslet.2014.10.012.
Jinxia Hu 1,  Miaomiao Niu 2,  Xueying Li 2,  Danyu Lu 2,  Jia Cui 2,  Weizhi Xu 2,  Gang Li 3,  Jun Zhan 2,  Hongquan Zhang 4
Affiliations

Affiliations

  • 1 Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Health Science Center, Beijing 100191, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China; Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China; Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264000, Shandong, China.
  • 2 Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Health Science Center, Beijing 100191, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China.
  • 3 Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China.
  • 4 Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Health Science Center, Beijing 100191, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Laboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China. Electronic address: [email protected].
PMID: 25448675 DOI: 10.1016/j.febslet.2014.10.012
Abstract

FRMD5 is a novel FERM domain-containing protein depicted in tumor progression. However, the mechanisms underlying FRMD5 inhibition of cell migration is largely unknown. Here, we show that FRMD5 regulates cell migration by interacting with Integrin β5 cytoplasmic tail and ROCK1 in human lung Cancer cells. FRMD5 promotes cell-matrix adhesion and cell spreading on vitronectin, and thus inhibits cell migration. Furthermore, FRMD5 interacts with ROCK1 and inhibits its activation that leads to the inhibition of Myosin light chain phosphorylation and the actin stress fiber formation. Taken together, these findings demonstrate that the putative tumor suppressive protein FRMD5 regulates tumor cell motility via a dual pathway involving FRMD5 binding to Integrin β5 tail and to ROCK1.

Keywords

Cell migration; FRMD5; Integrin β5; ROCK1.

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