2. Epigenetics
  3. Protein Arginine Deiminase
  4. PADI4_11

PADI4_11 is an isoform-selective, allosteric PADI4 activator with a Kd of 457 nM. PADI4_11 enters cells via active transport, does not induce cytotoxicity or membrane damage, and serves as a tool for investigating the regulation, function and cellular reprogramming of PADI4. PADI4_11 can be used in studies related to rheumatoid arthritis and ulcerative colitis.

For research use only. We do not sell to patients.

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PADI4_11

PADI4_11 Chemical Structure

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PADI4_11 Related Antibodies

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Description

PADI4_11 is an isoform-selective, allosteric PADI4 activator with a Kd of 457 nM. PADI4_11 enters cells via active transport, does not induce cytotoxicity or membrane damage, and serves as a tool for investigating the regulation, function and cellular reprogramming of PADI4. PADI4_11 can be used in studies related to rheumatoid arthritis and ulcerative colitis[1][2].

In Vitro

PADI4_11 binds selectively to calcium-bound and active-site-blocked calcium-bound recombinant hPADI4 with nanomolar affinity, but does not bind to calcium-free PADI4[1].
PADI4_11 (0.003-100 μM) allosterically activates recombinant PADI4 by reducing its calcium ion requirement, enhances the maximum activity under calcium ion-saturated conditions, and exhibits isozyme selectivity for PADI4[1].
PADI4_11 (100 nM-50 μM; 4 h) exhibits cell permeability in HaLo-GFP-mito HeLa cells, and its uptake is partially mediated by active transport mechanisms[1].
PADI4_11 (1-50 μM; 1 h) specifically activates PADI4 in hPADI4-stable mouse embryonic stem cells (mES cells) without additional stimulation, shows no off-target effect on mouse PADI4, and exhibits no signs of cytotoxicity[1].
PADI4_11 specifically binds to recombinant PADI4 in the calcium-bound active conformation, even when the active site is blocked, with a Kd value of 457 nM for the calcium-bound form[2].
PADI4_11 (100 nM-50 μM; 4 h) exhibits cell permeability in HaLo-GFP-mito HeLa cells, with a CP50 value of 3.1 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PADI4_11 Related Antibodies

Immunofluorescence[1]

Cell Line: hPADI4-stable mouse embryonic stem (mES) cells, wild-type mES cells
Concentration: 1-50 μM
Incubation Time: 1 h
Result: Induced dose-dependent increases in H3Cit intensity in hPADI4-stable mES cells, with an EC50 of 15.62 μM.
Showed no induction of H3Cit in wild-type mES cells (which only express mouse PADI4) at any tested concentration.
Did not increase intracellular calcium levels or cause cell membrane permeability/propidium iodide uptake at tested concentrations.
Molecular Weight

1596.81

Formula

C70H109N21O20S
Sequence

Tyr-Glu-Ser-Cys-Arg-Tyr-Arg-Gln-Val-Leu-Gln-Leu-NH2 (thioether bond:Tyr1-Cys4)
Sequence Shortening

YESCRYRQVLQL-NH2 (thioether bond:Tyr1-Cys4)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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PADI4_11 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PADI4_11Protein Arginine DeiminasePeptidylarginine Deiminaserheumatoid arthritisPADI4wild-type mES cellsulcerative colitisHaLo-GFP-mito HeLa cellshPADI4-stable mES cellspeptidylarginine deiminase IVhistone H3 citrullinationInhibitorinhibitorinhibit

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