Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens

  • J Ethnopharmacol. 2003 Feb;84(2-3):181-5. doi: 10.1016/s0378-8741(02)00318-5.
K M Park  1 ,  J S You ,  H Y Lee ,  N I Baek ,  J K Hwang
Affiliations
  • 1. Department of Biotechnology and Bioproducts Research Center, Yonsei University, Seoul 120-749, South Korea.
PMID: 12648813 DOI: 10.1016/s0378-8741(02)00318-5
Abstract

Kuwanon G was isolated from the ethyl acetate fraction of methanol extract of Morus alba and its structure was elucidated by 13C-NMR, 1H-NMR and FAB-MS. Antibacterial activity of kuwanon G was investigated by the minimum inhibitory concentration (MIC) test and the viable cell count method. MIC of kuwanon G against Streptococcus mutans causing dental caries was determined to be 8.0 microg/ml. The bactericidal test showed that kuwanon G completely inactivated S. mutans at the concentration 20 microg/ml in 1 min. Kuwanon G also significantly inhibited the growth of Other cariogenic bacteria such as Streptococcus sobrinus and Streptococcus sanguis, and Porpyromonas gingivalis causing periodontitis. Transmission electron microscopy (TEM) of kuwanon G treated cells demonstrated remarkable morphological damage of the cell wall and condensation of the cytoplasm.

Products