Hepatoprotective triterpenes from traditional Tibetan medicine Potentilla anserina

  • Phytochemistry. 2014 Jun;102:169-81. doi: 10.1016/j.phytochem.2014.03.002.
Toshio Morikawa  1 ,  Kiyofumi Ninomiya  1 ,  Katsuya Imura  1 ,  Takahiro Yamaguchi  1 ,  Yoshinori Akagi  1 ,  Masayuki Yoshikawa  1 ,  Takao Hayakawa  1 ,  Osamu Muraoka  2
Affiliations
  • 1. Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan.
  • 2. Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan. Electronic address: [email protected].
PMID: 24697904 DOI: 10.1016/j.phytochem.2014.03.002
Abstract

A methanol extract from the tuberous roots of Potentilla anserina (Rosaceae) exhibited hepatoprotective effects against d-galactosamine (d-GalN)/lipopolysaccharide-induced liver injuries in mice. Six triterpene 28-O-monoglucopyranosyl esters, potentillanosides A-F, were isolated from the extract along with 32 known compounds, including 15 Triterpenes. The structures of potentillanosides A-F were determined on the basis of spectroscopic properties and chemical evidence. Four ursane-type triterpene 28-O-monoglycosyl esters, potentillanoside A (IC50=46.7μM), 28-O-β-d-glucopyranosyl pomolic acid (IC50=9.5μM), rosamutin (IC50=35.5μM), and kaji-ichigoside F1 (IC50=14.1μM), inhibited d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes. Among these four Triterpenes, potentillanoside A, rosamutin, and kaji-ichigoside F1 exhibited in vivo hepatoprotective effects at doses of 50-100mg/kg, p.o. The mode of action was ascribable to the reduction in cytotoxicity caused by d-GalN.

Keywords
Hepatoprotective activity; Potentilla anserina; Potentillanoside; Rosaceae; Traditional Tibetan medicine; Ursane-type triterpene 28-O-monoglucopyranosyl ester.
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