A red-shifted fluorescent substrate for aldehyde dehydrogenase

  • Nat Commun. 2014 Apr 23:5:3662. doi: 10.1038/ncomms4662.
Il Minn  1 ,  Haofan Wang  1 ,  Ronnie C Mease  2 ,  Youngjoo Byun  3 ,  Xing Yang  2 ,  Julia Wang  4 ,  Steven D Leach  4 ,  Martin G Pomper  5
Affiliations
  • 1. 1] Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA [2].
  • 2. Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA.
  • 3. College of Pharmacy, Korea University, 2511 Sejong-ro, Jochiwon-eup, Sejong 339-700, South Korea.
  • 4. Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine, Baltimore, Maryland 21205, USA.
  • 5. 1] Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA [2] Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland 21287, USA.
PMID: 24759454 DOI: 10.1038/ncomms4662
Abstract

Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

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