MUC1 aptamer-based near-infrared fluorescence probes for tumor imaging

  • Mol Imaging Biol. 2015 Feb;17(1):38-48. doi: 10.1007/s11307-014-0763-y.
Haiyan Chen  1 ,  Juan Zhao ,  Min Zhang ,  Haibo Yang ,  Yuxiang Ma ,  Yueqing Gu
Affiliations
  • 1. Department of Biomedical Engineering, School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009, China.
PMID: 25005106 DOI: 10.1007/s11307-014-0763-y
Abstract

Purpose: DNA aptamer (APT) is able to bind to Mucin 1 (MUC1) specifically. The possibility of APT acting as a moiety to construct tumor-targeting probes was investigated.

Procedures: A near-infrared (NIR) Fluorescent Dye (MPA) and polyethylene glycol (PEG) were conjugated to APT to form APT-MPA and APT-PEG-MPA. The successful synthesis of the two probes was characterized via thin layer chromatography (TLC) and optical spectra. The tumor-targeting efficacy of the probes was evaluated in detail at cell level and animal level, respectively.

Results: The results indicated that MPA and PEG were successfully coupled with APT. APT-based probes were mediated by Mucin 1 into tumor cells, and PEG-modified probe exhibited higher cell affinity.

Conclusions: The aptamer-based NIR fluorescent probes are promising candidates for tumor imaging and diagnosis.

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