Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

  • Virus Res. 2017 Jan 2;227:49-56. doi: 10.1016/j.virusres.2016.09.013.
Seung Mok Ryu  1 ,  Jaeyoung Kwon  2 ,  Young Hye Seo  1 ,  Eun Gyeong Song  3 ,  Seong Su Hong  4 ,  Beom Seok Kim  1 ,  Jin Sung Hong  5 ,  Ki Hyun Ryu  6 ,  Dongho Lee  7
Affiliations
  • 1. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
  • 2. Natural Constituents Research Center, Korea Institute of Science and Technology (KIST), Gangneung, 25451, Republic of Korea.
  • 3. Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea.
  • 4. Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
  • 5. Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chunchon, 24341, Republic of Korea.
  • 6. Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea. Electronic address: [email protected].
  • 7. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: [email protected].
PMID: 27686478 DOI: 10.1016/j.virusres.2016.09.013
Abstract

A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify Antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host Plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40μM.

Keywords
Antiviral agent; Brucea javanica; Capsicum annuum; Green fluorescent protein; Pepper mottle virus; Quassinoid.
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