Melanogenesis inhibitory pregnane glycosides from Cynanchum atratum

  • Bioorg Med Chem Lett. 2018 Apr 15;28(7):1252-1256. doi: 10.1016/j.bmcl.2018.01.004.
Qinghao Jin  1 ,  Xiang Hua Han  1 ,  Cheong-Yong Yun  1 ,  Chul Lee  1 ,  Jin Woo Lee  1 ,  Dongho Lee  2 ,  Mi Kyeong Lee  1 ,  Sang-Hun Jung  3 ,  Jin Tae Hong  1 ,  Youngsoo Kim  1 ,  Bang Yeon Hwang  4
Affiliations
  • 1. College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea.
  • 2. School of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
  • 3. College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea.
  • 4. College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea. Electronic address: [email protected].
PMID: 29526485 DOI: 10.1016/j.bmcl.2018.01.004
Abstract

Bioassay-guided fractionation of the methanolic extract from the roots of Cynanchum atratum has resulted in the isolation of three new pregnane glycosides (1-3) along with four known compounds (4-7). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for their potential to inhibit the melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Of these, compounds 4-7 dose-dependently inhibited the melanin production with the IC50 values ranging from 4 μM to 33 μM.

Keywords
Asclepiadaceae; Cynanchum atratum; Melanogenesis inhibitor; Pregnane glycosides; α-MSH.