Osteochondral Differentiation of Fluorescent Multireporter Cells on Zonally-Organized Biomaterials

  • Tissue Eng Part A. 2019 Mar;25(5-6):468-486. doi: 10.1089/ten.TEA.2018.0135.
Drew S Clearfield  1  2 ,  Xiaonan Xin  2 ,  Sumit Yadav  3 ,  David W Rowe  2 ,  Mei Wei  1
Affiliations
  • 1. 1 Department of Materials Science and Engineering, Institute of Materials Science, University of Connecticut, Storrs, Connecticut.
  • 2. 2 Center for Regenerative Medicine and Skeletal Development and School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut.
  • 3. 3 Department of Orthodontics, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut.
PMID: 30136616 DOI: 10.1089/ten.TEA.2018.0135
Abstract

This study represents significant advancement in the use of biomimetic scaffolds to direct zonal osteochondral tissue formation. We describe the use of a novel fluorescent reporter system that enables the real-time evaluation of cellular differentiation in a nondestructive manner. In this study, we use this tool to confirm the osteogenic and chondrogenic capabilities of our scaffold alongside control scaffolds, and use cryohistological methods to probe zone-specific differences in cell and tissue quality. We believe this approach can be widely adopted by Others for a variety of biomaterial and cell systems in the development of tissue engineered therapeutics.

Keywords
chondrogenesis; osteochondral; osteogenesis; reporter cell; zonal scaffold.
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