6-Gingerol attenuates ventilator-induced lung injury via anti-inflammation and antioxidative stress by modulating the PPARγ/NF-κBsignalling pathway in rats
- Int Immunopharmacol. 2021 Mar;92:107367. doi: 10.1016/j.intimp.2021.107367.
- 1. Department of Anesthesiology, Huazhong University of Science and Technology Union ShenZhen Hospital, ShenZhen, China; Department of Anesthesiology, The 6th Affiliated Hospital of Shenzhen University Health Science Center, ShenZhen, China.
- 2. Institute of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Anesthesiology, Subei People's Hospital, YangZhou 225001, China.
- 3. Department of General Medicine, Huazhong University of Science and Technology Union ShenZhen Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, ShenZhen, China.
- 4. Department of Anesthesiology, Yuebei People's Hospital, Shantou University Medical College, Shaoguan, Guangdong Province, China.
- 5. Institute of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: [email protected].
- 6. Department of Anesthesiology, Huazhong University of Science and Technology Union ShenZhen Hospital, ShenZhen, China; Department of Anesthesiology, The 6th Affiliated Hospital of Shenzhen University Health Science Center, ShenZhen, China. Electronic address: [email protected].
Although mechanical ventilation (MV) is indispensable to life-support therapy in critically ill patients, it may promote or aggravatelunginjury known asventilator-inducedlunginjury(VILI). 6-Gingerol is the principal ingredient of ginger with potential anti-inflammatory and antioxidant properties in various diseases. Nevertheless, the role and mechanism of 6-gingerol in the process of VILI has not been explicitly investigated. In the study, we found that pre-treatment with 6-gingerol significantly improved the histological changes and pulmonary oedema, inhibited neutrophil accumulation and the release of early pro-inflammatory cytokines and MPO, and reduced oxidative stress reactions after high MV. Moreover, 6-gingerol treatment also increased PPARγ expression and decreased NF-κB activation in rats subjected to high MV. Furthermore, GW9662, a specific PPARγ Inhibitor, was demonstrated to activatethe NF-κB pathway and cancele the protective role of 6-gingerol in VILI. This indicates that 6-gingerol exerted anti-inflammatory and antioxidative stress effects in VILI by activating PPARγ and inhibiting the NF-κBsignalling pathway.