Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte

  • Antioxidants (Basel). 2021 May 19;10(5):806. doi: 10.3390/antiox10050806.
Tae-Young Kim  1 ,  Ye Jin Kim  2 ,  Jonghwan Jegal  1 ,  Beom-Geun Jo  1 ,  Han-Seok Choi  2 ,  Min Hye Yang  1
Affiliations
  • 1. College of Pharmacy, Pusan National University, Busan 46241, Korea.
  • 2. C&D Research Team, R&D Strategy Center, Genuonesciences, Seoul 06800, Korea.
PMID: 34069696 DOI: 10.3390/antiox10050806
Abstract

This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in D. dasycarpus. Haplopine (12.5 and 25 μM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF, and G-CSF and the protein expressions of IL-6 and GM-CSF in TNF-α/INF-γ-stimulated HaCaT cells. In H2O2-induced Jukat T cells, haplopine (25 and 50 μM) suppressed the productions of proinflammatory cytokines (IL-4, IL-13, and COX-2) and increased the mRNA and protein expressions of oxidative stress defense Enzymes (SOD, CAT, and HO-1) in a concentration-dependent manner. In vivo, haplopine significantly attenuated the development of AD symptoms in 2,4-dinitrochlorobenzene (DNCB)-stimulated Balb/c mice, as evidenced by reduced clinical dermatitis scores, skin thickness measurements, mast cell infiltration, and serum IgE concentrations. These findings demonstrate that haplopine should be considered a novel anti-atopic agent with the potential to treat AD.

Keywords
2,4-dinitrochlorobenzene; Balb/c mice; HaCaT cells; Jurkat T cells; atopic dermatitis; haplopine.
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