Effect of Lactobacillus rhamnosus MN-431 Producing Indole Derivatives on Complementary Feeding-Induced Diarrhea Rat Pups Through the Enhancement of the Intestinal Barrier Function

  • Mol Nutr Food Res. 2022 Jan;66(2):e2100619. doi: 10.1002/mnfr.202100619.
Haiyue Niu  1 ,  Xiaohong Zhou  2 ,  Pimin Gong  3 ,  Yuehua Jiao  4 ,  Jiliang Zhang  1 ,  Yifan Wu  1 ,  Linzheng Lyu  1 ,  Cong Liang  1 ,  Shiwei Chen  1 ,  Xue Han  1 ,  Lanwei Zhang  3
Affiliations
  • 1. School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, 150001, China.
  • 2. Qingdao Central Hospital, Qingdao, 266042, China.
  • 3. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
  • 4. Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150040, China.
PMID: 34806832 DOI: 10.1002/mnfr.202100619
Abstract

Scope: Many infants suffer from complementary feeding-induced diarrhea (CFID). Studies have shown that intestinal microbes can enhance the intestinal barrier and prevent diarrhea by producing indole derivatives that promote pregnane X receptor (PXR) expression.

Methods and results: In this study, the indole test and determination of the PXR concentration are performed on tryptophan broth cultures of 320-suspected Lactobacillus and Enterococcus strains. Four strains that produce indole derivatives that promote the expression of PXR are screened as potential functional probiotics. Both Lactobacillus rhamnosus MN-431 (L. rhamnosus MN-431) and Lactobacillus oris FN-448 (L. oris FN-448) can colonize the intestine of rat pups, and L. rhamnosus MN-431 can significantly decrease the incidence of diarrhea and intestinal permeability in rat pups. Using real-time qPCR and the analysis of the intestinal morphology using immunohistochemistry, it is observed that the metabolized tryptophan from L. rhamnosus MN-431 can reduce small intestinal mucosal damage by stimulating PXR/NF-κB signaling and activating PXR and Aryl Hydrocarbon Receptor. The intestinal barrier is also enhanced by promoting the expression of tight junction proteins such as Occludin and zonula occludens-1 in baby rats.

Conclusion: The results demonstrate that L. rhamnosus MN-431 can metabolize tryptophan to prevent infantile CFID by promoting the expression of PXR.

Keywords
L. rhamnosus MN-431; complementary feeding-induced diarrhea; indole derivatives; pregnane X receptor; tryptophan metabolism.
Products