The Role of NO Synthase, MPT pore, and Protein Kinase A in the Cardioprotective Effect of the Opioid Receptor Agonist Deltorphin II

  • Bull Exp Biol Med. 2023 May 10. doi: 10.1007/s10517-023-05784-4.
A V Mukhomedzyanov  1 ,  S V Popov  2 ,  L N Maslov  2 ,  V N Azev  3 ,  E R Diez  4
Affiliations
  • 1. Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia. [email protected].
  • 2. Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
  • 3. Branch of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
  • 4. Instituto de Fisiología, FCM - UNCuyo, IMBECU - CONICET-UNCuyo, Mendoza, Argentina.
PMID: 37160797 DOI: 10.1007/s10517-023-05784-4
Abstract

In male Wistar rats, coronary occlusion (45 min) and reperfusion (120 min) were modeled. Selective δ2-opioid receptor agonist (deltorphin II, 0.12 mg/kg) was administered intravenously 5 min before reperfusion; NO Synthase Inhibitor (L-NAME, 10 mg/kg), MPT pore blocker (atractyloside, 5 mg/kg), and protein kinase A inhibitor (H-89, 10 μg/kg) were administered intravenously 10 min before reperfusion. Deltorphin II administered before reperfusion led to a 2-fold decrease in the infarct size. The infarct-limiting effect of deltorphin II was associated with blockade of MPT pore. Protein kinase A and NO Synthase were not involved in the cardioprotective effect of deltorphin II.

Keywords
MPT pore; heart; ischemia/reperfusion; opioid receptors; protein kinase A.
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