The plant immune receptor SNC1 monitors helper NLRs targeted by a bacterial effector

  • Cell Host Microbe. 2023 Nov 8;31(11):1792-1803.e7. doi: 10.1016/j.chom.2023.10.006.
Ming-Yu Wang  1 ,  Jun-Bin Chen  1 ,  Rui Wu  2 ,  Hai-Long Guo  3 ,  Yan Chen  1 ,  Zhen-Ju Li  1 ,  Lu-Yang Wei  1 ,  Chuang Liu  1 ,  Sheng-Feng He  1 ,  Mei-Da Du  1 ,  Ya-Long Guo  4 ,  You-Liang Peng  3 ,  Jonathan D G Jones  5 ,  Detlef Weigel  6 ,  Jian-Hua Huang  7 ,  Wang-Sheng Zhu  8
Affiliations
  • 1. Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Ministry of Agriculture and Rural Affairs, and College of Plant Protection, State Key Laboratory of Maize Bio-breeding, China Agricultural University, Beijing 100193, China.
  • 2. Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany.
  • 3. Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs, and College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • 4. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
  • 5. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.
  • 6. Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany; Institute for Bioinformatics and Medical Informatics, University of Tübingen, Tübingen, Germany.
  • 7. The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: [email protected].
  • 8. Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Ministry of Agriculture and Rural Affairs, and College of Plant Protection, State Key Laboratory of Maize Bio-breeding, China Agricultural University, Beijing 100193, China. Electronic address: [email protected].
PMID: 37944492 DOI: 10.1016/j.chom.2023.10.006
Abstract

Plants deploy intracellular receptors to counteract pathogen effectors that suppress cell-surface-receptor-mediated immunity. To what extent pathogens manipulate intracellular receptor-mediated immunity, and how Plants tackle such manipulation, remains unknown. Arabidopsis thaliana encodes three similar ADR1 class helper nucleotide-binding domain leucine-rich repeat receptors (ADR1, ADR1-L1, and ADR1-L2), which are crucial in plant immunity initiated by intracellular receptors. Here, we report that Pseudomonas syringae effector AvrPtoB suppresses ADR1-L1- and ADR1-L2-mediated cell death. ADR1, however, evades such suppression by diversifying into two ubiquitination sites targeted by AvrPtoB. The intracellular sensor SNC1 interacts with and guards the CCR domains of ADR1-L1/L2. Removal of ADR1-L1/L2 or delivery of AvrPtoB activates SNC1, which then signals through ADR1 to trigger immunity. Our work elucidates the long-sought-after function of SNC1 in defense, and also how Plants can use dual strategies, sequence diversification, and a multi-layered guard-guardee system, to counteract pathogen's attack on core immunity functions.

Keywords
ADR1s; E3 ubiquitin ligase; Pseudomonas effector; SNC1; effector-triggered immunity; helper NLRs; plant and microbe interactions; plant immunity.
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