Copy Link Print Information

ADH5 Protein, Human

Customer Review

Based on 1 Customer Validation

The ADH5 protein catalyzes the oxidation of long-chain primary alcohols and S-(hydroxymethyl) glutathione. It also oxidizes long-chain omega-hydroxy fatty acids, producing intermediate aldehydes and end products. However, it is ineffective in oxidizing ethanol. ADH5 is crucial for clearing cytotoxic formaldehyde, which causes DNA damage. ADH5 Protein, Human is the recombinant human-derived ADH5 protein, expressed by E. coli , with no tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • ADH5 Protein, Human Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The ADH5 protein catalyzes the oxidation of long-chain primary alcohols and S-(hydroxymethyl) glutathione. It also oxidizes long-chain omega-hydroxy fatty acids, producing intermediate aldehydes and end products. However, it is ineffective in oxidizing ethanol. ADH5 is crucial for clearing cytotoxic formaldehyde, which causes DNA damage. ADH5 Protein, Human is the recombinant human-derived ADH5 protein, expressed by E. coli , with no tag.

Background

ADH5, a Class-III alcohol dehydrogenase, plays a pivotal role in cellular detoxification processes by catalyzing the oxidation of long-chain primary alcohols and S-(hydroxymethyl) glutathione. Additionally, ADH5 is involved in the oxidation of long-chain omega-hydroxy fatty acids, such as 20-hydroxyeicosatetraenoic acid (20-HETE), yielding both the intermediate aldehyde, 20-oxoarachidonate, and the final product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Despite its classification as an alcohol dehydrogenase, ADH5 exhibits low efficiency in oxidizing ethanol. Notably, ADH5 is crucial for cellular defense against formaldehyde, a cytotoxic and carcinogenic metabolite, contributing to the clearance of this substance and preventing potential DNA damage.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P11766 (M1-I374)

  • Gene ID
    128
  • Molecular Construction
    • N-term
    • ADH5 (M1-I374)
      Accession # P11766
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ADH5; S-Nitrosoglutathione Reductase; Prev. FDH; Alcohol Dehydrogenase 5; ADHX; HEL-S-60p; Glutathione-Dependent Formaldehyde Dehydrogenase; GSH-FDH; S-(Hydroxymethyl)Glutathione Dehydrogenase; FALDH; Alcohol Dehydrogenase Class-III; Alcohol Dehydrogenase

  • AA Sequence

    MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

  • Predicted Molecular Mass

    39.7 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • ADH5 Protein, Human Product Image
    1/16
  • ADH5 Protein, Human Product Image