1. Recombinant Proteins
  2. Others
  3. Calsequestrin 1 Protein, Human

Calsequestrin 1 Protein, Human

Cat. No.: HY-P75459
COA Handling Instructions

Calsequestrin 1 protein is a high-capacity calcium-binding protein that serves as an internal calcium store in muscles. Calsequestrin 1 Protein, Human is the recombinant human-derived Calsequestrin 1 protein, expressed by E. coli , with tag free. The total length of Calsequestrin 1 Protein, Human is 362 a.a., with molecular weight of ~52 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $45 In-stock
10 μg $75 In-stock
50 μg $200 In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Calsequestrin 1 protein is a high-capacity calcium-binding protein that serves as an internal calcium store in muscles. Calsequestrin 1 Protein, Human is the recombinant human-derived Calsequestrin 1 protein, expressed by E. coli , with tag free. The total length of Calsequestrin 1 Protein, Human is 362 a.a., with molecular weight of ~52 kDa.

Background

Calsequestrin 1, a high-capacity calcium-binding protein, serves as an internal calcium store in muscle. Clusters of acidic residues at the protein surface, particularly at the subunit interface, facilitate the binding of approximately 80 Ca(2+) ions. This protein plays a pivotal role in regulating the release of lumenal Ca(2+) through the calcium release channel RYR1, thereby contributing to the initiation of muscle contraction. Additionally, Calsequestrin 1 acts as a negative regulator of store-operated Ca(2+) entry (SOCE) activity. Existing as a monomer that increases in response to intracellular calcium depletion, it can also form homodimers and higher-order homooligomers, with Ca(2+) ions promoting oligomerization. Calsequestrin 1 interacts with STIM1, preferentially in its monomeric form, and this interaction intensifies following intracellular calcium depletion. Furthermore, it interacts with ASPH and TRDN, highlighting its involvement in diverse cellular processes related to calcium homeostasis.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P31415 (Q35-D396)

Gene ID

844  [NCBI]

Molecular Construction
N-term
CASQ1 (Q35-D396)
Accession # P31415
C-term
Synonyms
Calsequestrin-1; Calmitine; CASQ1; CASQ
AA Sequence

QEGLDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPEDDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDAAEEFHPYIPFFATFDSKVAKKLTLKLNEIDFYEAFMEEPVTIPDKPNSEEEIVNFVEEHRRSTLRKLKPESMYETWEDDMDGIHIVAFAEEADPDGFEFLETLKAVAQDNTENPDLSIIWIDPDDFPLLVPYWEKTFDIDLSAPQIGVVNVTDADSVWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDDDDDDD

Molecular Weight

Approximately 52 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Calsequestrin 1 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Calsequestrin 1 Protein, Human
Cat. No.:
HY-P75459
Quantity:
MCE Japan Authorized Agent: