CD45 Protein, Mouse (sf9)
Based on 1 Customer Validation
CD45 Protein, a versatile member of the protein tyrosine phosphatase family, displays heparan sulfate proteoglycan and heparin binding activity, as well as protein tyrosine phosphatase activity. It plays a crucial role in lymphocyte differentiation, macromolecule metabolic processes, and signal transduction regulation. CD45 is associated with diseases such as systemic lupus erythematosus, hepatitis C, multiple sclerosis, and severe combined immunodeficiency. CD45 Protein, Mouse (sf9) is the recombinant mouse-derived CD45 protein, expressed by Sf9 insect cells , with tag free.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
CD45 Protein, a versatile member of the protein tyrosine phosphatase family, displays heparan sulfate proteoglycan and heparin binding activity, as well as protein tyrosine phosphatase activity. It plays a crucial role in lymphocyte differentiation, macromolecule metabolic processes, and signal transduction regulation. CD45 is associated with diseases such as systemic lupus erythematosus, hepatitis C, multiple sclerosis, and severe combined immunodeficiency. CD45 Protein, Mouse (sf9) is the recombinant mouse-derived CD45 protein, expressed by Sf9 insect cells , with tag free.
Receptor-type tyrosine-protein phosphatase C (CD45), a member of the protein tyrosine phosphatase family, exhibits versatile functions, including heparan sulfate proteoglycan binding activity, heparin binding activity, and protein tyrosine phosphatase activity. It is intricately involved in key processes such as lymphocyte differentiation, positive regulation of macromolecule metabolic processes, and regulation of signal transduction. CD45 acts upstream in lymphocyte-related pathways, including differentiation and activation, while being prominently located in the external side of the plasma membrane, focal adhesion, and membrane raft. Widely expressed in various structures such as the 3rd branchial arch, alimentary system, cardiovascular system, hemolymphoid system, and placenta, CD45's significance is underscored by its association with conditions like systemic lupus erythematosus. The human ortholog of this gene, PTPRC (protein tyrosine phosphatase receptor type C), is implicated in hepatitis C, multiple sclerosis, and severe combined immunodeficiency, highlighting its pivotal role in immune-related disorders[1][2][3].
1.The specific activity was determined to be 9168 nmol/min/mg using p-nitrophenyl phosphate as substrate.
2.Immobilized mouse PTPRC at 10 μg/ml (100 μl/well) can bind biotinylated human Galectin-1 with a linear range of 0.31-2.5 μg/ml.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag Tag Free
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Accession
P06800-4 (R594-S1293)
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Molecular Construction
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N-term
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CD45 (R594-S1293)
Accession # P06800-4 -
C-term
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Protein Length
Partial
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Synonyms
PTPRC; Leukocyte Common Antigen; Prev. CD45; CD45 Antigen; T200; L-CA; Receptor-Type Tyrosine-Protein Phosphatase C; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; GP180; Protein Tyrosine Phosphatase, Receptor Type, C; CD45R; Protein-Tyrosine
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AA Sequence
RKKRSSNLDEQQELVERDDEKQLMDVEPIHSDILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKDARKPHNQNKNRYVDILPYDYNRVELSEINGDAGSTYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEEGTRAFKDIVVTINDHKRCPDYIIQKLNVAHKKEKATGREVTHIQFTSWPDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLEGLEAEGKVDVYGYVVKLRRQRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHSCLHNMKKRDPPSDPSPLEAEYQRLPSYRSWRTQHIGNQEENKKKNRNSNVVPYDFNRVPLKHELEMSKESEPESDESSDDDSDSEETSKYINASFVMSYWKPEMMIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELVNGDQEVCAQYWGEGKQTYGDMEVEMKDTNRASAYTLRTFELRHSKRKEPRTVYQYQCTTWKGEELPAEPKDLVSMIQDLKQKLPKASPEGMKYHKHASILVHCRDGSQQTGLFCALFNLLESAETEDVVDVFQVVKSLRKARPGVVCSYEQYQFLYDIIASIYPAQNGQVKKTNSQDKIEFHNEVDGGKQDANCVRPDGPLNKAQEDSRGVGTPEPTNSAEEPEHAANGSASPAPTQSS
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Predicted Molecular Mass
81 kDa
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Molecular Weight
Approximately 93 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, 3 mM DTT, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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