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Clumping factor B Protein, S. aureus (His, SUMO)

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Clumping factor B (ClfB) is a cell surface-associated protein implicated in virulence, playing a crucial role in bacterial pathogenicity by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen. Its significance lies in inducing the formation of bacterial clumps, a mechanism that contributes to the pathogenicity and virulence of the bacteria. Clumping factor B Protein, S. aureus (His, SUMO) is the recombinant Staphylococcus aureus-derived Clumping factor B protein, expressed by E. coli , with N-6*His-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Staphylococcus aureus
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Clumping factor B Protein, S. aureus (His, SUMO) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Clumping factor B (ClfB) is a cell surface-associated protein implicated in virulence, playing a crucial role in bacterial pathogenicity by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen. Its significance lies in inducing the formation of bacterial clumps, a mechanism that contributes to the pathogenicity and virulence of the bacteria. Clumping factor B Protein, S. aureus (His, SUMO) is the recombinant Staphylococcus aureus-derived Clumping factor B protein, expressed by E. coli , with N-6*His-SUMO labeled tag.

Background

Clumping factor B (ClfB) is a cell surface-associated protein crucial for bacterial virulence, as it plays a pivotal role in promoting bacterial attachment. It specifically binds to both alpha- and beta-chains of human fibrinogen, facilitating the formation of bacterial clumps. This interaction is significant in the context of host-pathogen interactions, as it enhances the adherence of bacteria to fibrinogen, contributing to the pathogenicity of the organism. The ability of ClfB to induce bacterial clumps suggests its involvement in the early stages of infection and highlights its potential as a target for therapeutic interventions aimed at disrupting bacterial adherence and virulence (Reference: Information provided)

Technical Parameters

  • Species Staphylococcus aureus
  • Source E. coli
  • Tag N-SUMO;N-6*His
  • Accession

    Q6GDH2 (S45-N542)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • Clumping factor B (S45-N542)
      Accession # Q6GDH2
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SAR2709; clfB; Fibrinogen-binding protein B; Fibrinogen receptor B; Clumping factor B

  • AA Sequence

    SEQSNDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKTMSTQTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATNSELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDKVTASDFKLEKTAFDPNQSGNTFMAANFKVTGQVKSGDYFTAKLPDSVTGNGDVDYSNSNNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKAPKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQTVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADPNDSNLKEVTGEFKDKISYKYDNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQENIDPATGKDYSIFGWNNENVVRYGGGSADGDSAVN

  • Predicted Molecular Mass

    69.8 kDa

  • Molecular Weight

    Approximately 80 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1.0 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Clumping factor B Protein, S. aureus (His, SUMO) Product Image