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ITM2A Protein, Mouse (Cell-Free, His)

Cat. No.: HY-P702339
Handling Instructions

The ITM2A protein is expressed in the mandibular condyle, bone, and hair follicles, and is particularly robust in osteogenic tissues, suggesting involvement in bone development and maintenance, emphasizing its role in osteogenesis. Expression in hair follicles suggests additional functions in skin-related processes. ITM2A Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived ITM2A protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ITM2A Protein, Mouse (Cell-Free, His) is 263 a.a., with molecular weight of 31.2 kDa.

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Description

The ITM2A protein is expressed in the mandibular condyle, bone, and hair follicles, and is particularly robust in osteogenic tissues, suggesting involvement in bone development and maintenance, emphasizing its role in osteogenesis. Expression in hair follicles suggests additional functions in skin-related processes. ITM2A Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived ITM2A protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ITM2A Protein, Mouse (Cell-Free, His) is 263 a.a., with molecular weight of 31.2 kDa.

Background

ITM2A protein exhibits expression in mandibular condyles, bone, and hair follicles, with particularly robust expression in osteogenic tissues such as neonatal calvaria, paws, tail, and skin. The presence of ITM2A in these specific tissues suggests its potential involvement in skeletal development and maintenance, emphasizing its role in osteogenesis. Furthermore, its expression in hair follicles hints at additional functions in skin-related processes. The distinct spatial expression pattern of ITM2A underscores its significance in various tissues, implicating its participation in molecular events related to bone development and homeostasis, as well as potential contributions to skin and hair biology.

Species

Mouse

Source

E. coli Cell-free

Tag

N-10*His

Accession

Q61500 (M1-E263)

Gene ID

16431

Molecular Construction
N-term
10*His
ITM2A (M1-E263)
Accession # Q61500
C-term
Synonyms
Integral membrane protein 2A; Protein E25
AA Sequence

MVKIAFNTPTAVQKEEARQDVEALVSRTVRAQILTGKELRVVPQEKDGSSGRCMLTLLGLSFILAGLIVGGACIYKYFMPKSTIYHGEMCFFDSEDPVNSIPGGEPYFLPVTEEADIREDDNIAIIDVPVPSFSDSDPAAIIHDFEKGMTAYLDLLLGNCYLMPLNTSIVMTPKNLVELFGKLASGKYLPHTYVVREDLVAVEEIRDVSNLGIFIYQLCNNRKSFRLRRRDLLLGFNKRAIDKCWKIRHFPNEFIVETKICQE

Molecular Weight

31.2 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ITM2A Protein, Mouse (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ITM2A Protein, Mouse (Cell-Free, His)
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HY-P702339
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