MCR-1 protein, E.coli (364a.a, His-SUMO)
Based on 1 Customer Validation
The MCR-1 protein may catalyze the addition of a phosphoethanolamine moiety to lipid A, which is associated with polymyxin resistance. This modification observed in E. coli expressing MCR-1 increased the minimum inhibitory concentration (MIC) of colistin and polymyxin B. MCR-1 protein, E.coli (364a.a, His-SUMO) is the recombinant E. coli-derived MCR-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The MCR-1 protein may catalyze the addition of a phosphoethanolamine moiety to lipid A, which is associated with polymyxin resistance. This modification observed in E. coli expressing MCR-1 increased the minimum inhibitory concentration (MIC) of colistin and polymyxin B. MCR-1 protein, E.coli (364a.a, His-SUMO) is the recombinant E. coli-derived MCR-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
The MCR-1 protein is likely responsible for catalyzing the addition of a phosphoethanolamine moiety to lipid A, a modification associated with polymyxin resistance. This modification plays a crucial role in conferring resistance to polymyxin-type antibiotics, as evidenced by increased minimal inhibitory concentrations (MIC) of colistin and polymyxin B in E. coli expressing MCR-1. The pHNSHP45 plasmid, carrying the mcr-1 gene, exhibits efficient transferability to other E. coli strains and significantly elevates polymyxin MIC. Notably, this resistance may not necessitate selective pressure for maintenance within the cell. When introduced into other bacterial species like K. pneumoniae or P. aeruginosa, the plasmid similarly enhances polymyxin MIC. In a murine thigh infection study using an mcr-1-encoding plasmid from a human patient, the plasmid provides in vivo protection against colistin. These findings underscore the crucial role of MCR-1 in mediating resistance to polymyxin antibiotics in various bacterial strains.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
A0A0R6L508 (H178-R541)
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Gene ID55632978
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Molecular Construction
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N-term
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6*His-SUMO
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MCR-1 (H178-R541)
Accession # A0A0R6L508 -
C-term
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Protein Length
Periplasmic Domain
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Synonyms
Phosphatidylethanolamine transferase Mcr-1; EC:2.7.8.43; Polymyxin resistance protein MCR-1; mcr1; mcr-1; APZ14_31440
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AA Sequence
HYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR
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Predicted Molecular Mass
56.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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