PAK4 Protein, Human (His)

PAK4 is an important serine/threonine kinase that affects cytoskeletal regulation, cell migration, growth, and survival through multiple pathways. Activation triggered by various effectors induces autophosphorylation, targeting proteins such as SSH1 and LIMK1 to regulate cofilin activity and stabilize actin filaments. PAK4 Protein, Human (His) is the recombinant human-derived PAK4, expressed by E. coli , with N-His labeled tag. ,

PAK4, a serine/threonine protein kinase, exerts its influence across diverse signaling pathways, impacting cytoskeleton regulation, cell migration, growth, proliferation, and cell survival. Activation triggered by various effectors, such as growth factor receptors or active CDC42 and RAC1, induces a conformational change and subsequent autophosphorylation on multiple serine and/or threonine residues. PAK4's phosphorylation targets include the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. This results in decreased cofilin activity, promoting the stabilization of actin filaments. Additionally, PAK4 phosphorylates LIMK1, another kinase that inhibits cofilin activity. By phosphorylating integrin beta5/ITGB5, PAK4 regulates cell motility, while its phosphorylation of ARHGEF2 activates the downstream target RHOA, contributing to the regulation of focal adhesion assembly and actin stress fibers. PAK4 plays a crucial role in cell survival by phosphorylating the BCL2 antagonist of cell death, BAD. Alternatively, it inhibits apoptosis by preventing the binding of caspase-8 to death domain receptors, acting in a kinase-independent manner. Furthermore, PAK4 influences cell-cycle progression by controlling the levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

The activity of PAK4 is verified by the ADP-Glo method: Incubate PAK4 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.

Human

E. coli

N-His

O96013-1 (M1-R591)

10298

Full Length of Isoform-1

65.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.