PpsA Protein, E.coli (His-SUMO)
The PpsA protein plays a crucial role in cellular metabolism by catalyzing the conversion of pyruvate to phosphoenolpyruvate, a key step in glycolysis and gluconeogenesis. This enzyme activity is the basis for the interconversion of metabolic intermediates and contributes to the regulation of intracellular energy production and utilization. PpsA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived PpsA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
Biological Activity
The PpsA protein plays a crucial role in cellular metabolism by catalyzing the conversion of pyruvate to phosphoenolpyruvate, a key step in glycolysis and gluconeogenesis. This enzyme activity is the basis for the interconversion of metabolic intermediates and contributes to the regulation of intracellular energy production and utilization. PpsA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived PpsA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
The PpsA protein is an enzyme that plays a crucial role in glycolysis and gluconeogenesis by catalyzing the phosphorylation of pyruvate to phosphoenolpyruvate. This enzymatic activity represents a pivotal step in the interconversion of metabolic intermediates, facilitating the entry of pyruvate into gluconeogenic pathways or its progression through glycolysis. The phosphorylation of pyruvate by PpsA is a reversible reaction and contributes to the regulation of the overall flux of carbon through these metabolic pathways. This enzyme is essential for maintaining cellular energy homeostasis and is integral to the fine-tuning of metabolic processes in response to the cell's energetic needs. Understanding the functions of PpsA provides valuable insights into the dynamic regulation of central carbon metabolism.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
P23538 (S2-K792)
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Gene ID946209 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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PpsA (S2-K792)
Accession # P23538 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Phosphoenolpyruvate synthase; EC:2.7.9.2; PEP synthase; Pyruvate; ppsA; water dikinase; pps; JW1692; b1702
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AA Sequence
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK
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Predicted Molecular Mass
103.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)