RAB2 Protein, Mouse (His)
Based on 1 Customer Validation
Rab2A belongs to the Rab family, it is a small molecular weight guanosine triphosphatases (GTPases) and membrane-bound protein which involved in GTP binding and hydrolysis and are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes, Rab2A is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex as well. Rab2A also regulates the compacted morphology of the Golgi. RAB2 Protein, Mouse (His) is the recombinant mouse-derived RAB2 protein, expressed by E. coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Rab2A belongs to the Rab family, it is a small molecular weight guanosine triphosphatases (GTPases) and membrane-bound protein which involved in GTP binding and hydrolysis and are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes, Rab2A is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex as well. Rab2A also regulates the compacted morphology of the Golgi. RAB2 Protein, Mouse (His) is the recombinant mouse-derived RAB2 protein, expressed by E. coli , with N-His labeled tag.
Rab2A belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis and are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rab2A is a membrane-bound protein and a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Rab2A also regulates the compacted morphology of the Golgi[1][2].
Measured in a cell proliferation assay using Hela cells. The ED50 this effect is 2.70 ng/mL, corresponding to a specific activity is 3.70×105 units/mg.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
Q0PD65 (M1-C212)
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Molecular Construction
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N-term
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His
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RAB2 (M1-C212)
Accession # Q0PD65 -
C-term
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Protein Length
Full Length
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Synonyms
RAB2A; Ras-Related Protein Rab-2A; Prev. RAB2; LHX; RAB2, Member RAS Oncogene Family; RAB2A, Member RAS Oncogene Family; Small GTP Binding Protein RAB2A
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AA Sequence
MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNASHGSNQGGQQAGGGCC
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Molecular Weight
Approximately 23-25 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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