SNAP-alpha Protein, Human (His)
Based on 1 Customer Validation
SNAP-α proteins are critical in vesicle trafficking, mediating dynamic exchange between the endoplasmic reticulum and the Golgi apparatus. It cooperates with GNA12 to localize CDH5 to the plasma membrane. SNAP-alpha Protein, Human (His) is the recombinant human-derived SNAP-alpha protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
SNAP-α proteins are critical in vesicle trafficking, mediating dynamic exchange between the endoplasmic reticulum and the Golgi apparatus. It cooperates with GNA12 to localize CDH5 to the plasma membrane. SNAP-alpha Protein, Human (His) is the recombinant human-derived SNAP-alpha protein, expressed by E. coli , with N-6*His labeled tag.
SNAP-alpha Protein stands as an indispensable player in vesicular transport, facilitating the dynamic exchange between the endoplasmic reticulum and the Golgi apparatus. Its collaboration with GNA12 extends its influence, promoting the localization of CDH5 to the plasma membrane. Moreover, SNAP-alpha engages in intricate protein interactions, forming complexes with PRKCABP and participating in the disruption of the interaction between GRIA2 and PRKCABP, consequently driving the internalization of GRIA2. Within a sophisticated SNARE-like assembly, SNAP-alpha takes its place alongside ZW10, USE1L, RINT1, STX18, and NAPA, revealing its integral role in membrane fusion events. This versatile protein further expands its interaction repertoire by engaging with VAMP8, VTI1A, and STX12. The complex interplay of SNAP-alpha in diverse cellular processes underscores its significance as a key orchestrator in the intricate landscape of intracellular transport and membrane dynamics.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
P54920 (M1-R295)
-
Molecular Construction
-
N-term
-
6*His
-
SNAP-alpha (M1-R295)
Accession # P54920 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
NAPA; N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha; NSF Attachment Protein Alpha; Alpha Soluble NSF Attachment Protein; N-Ethylmaleimide-Sensitive Factor Attachment Protein, Alpha; Alpha SNAP; Alpha-Soluble NSF Attachment Protein; Alpha-SNAP
-
AA Sequence
MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR
-
Molecular Weight
Approximately 35 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
