SAR-443809

SAR-443809 (Human FB: 6-500 nM; human FBb: 0.4-30 nM; SAR443809 Fab: 3.7-300 nM; 25°C during assay) binds with high affinity to human factor Bb (K_D = 7.3 nM) and cynomolgus factor Bb (K_D = 61 nM), and its Fab fragment binds to the active alternative pathway C3 convertase (C3bBb) with high affinity (K_D = 0.5 nM)^[1].
SAR-443809 (50 minutes at 37°C) inhibits alternative pathway-mediated hemolysis of rabbit erythrocytes with an IC₅₀ of 28.45 μg/mL^[1].
SAR-443809 (50 minutes at 37°C) inhibits alternative pathway-mediated C3b deposition on rabbit erythrocytes with an apparent IC₅₀ of 33.8 μg/mL^[1].
SAR-443809 potently and selectively inhibits the human complement alternative pathway (IC₅₀ = 8.47 μg/mL) and cynomolgus monkey complement alternative pathway (IC₅₀ = 15.72 μg/mL) by blocking cleavage of C3 and factor B, without inhibiting the classical or lectin pathways^[1].
SAR-443809 selectively binds to human factor Bb (EC₅₀ = 30.9 ng/mL) and shows no specific binding to other tested plasma serine proteases, including most complement system enzymes^[1].
SAR-443809 (100 μg/mL; 30 minutes at 37°C) inhibits unregulated fluid-phase C3 cleavage in factor H-depleted human serum, preventing consumption of C3 via alternative pathway tickover^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.