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Nitric oxide monitoring

" in MedChemExpress (MCE) Product Catalog:

By Targets:	NO Synthase (1) Fluorescent Dye (3)
By Research Areas:	Cancer (1) Cardiovascular Disease (1)

Inhibitors & Agonists

Fluorescent Dyes

Targets Recommended: NO Synthase

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D2966

TMR-NO-BG	Fluorescent Dye	Others
TMR-NO-BG is a hybrid probe (Ex/Em: 557 nm/571 nm). TMR-NO-BG covalently connects the tetramethylrhodamine (TMR) lactone NO sensing unit with the SNAP-tag reactive benzyl guanine (BG) for achieving subcellular-specific nitric oxide (NO) detection .

HY-D3250

PYSNO	Fluorescent Dye NO Synthase	Cardiovascular Disease
PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λ_em=515-565 nm, λ_ex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases .

HY-161476

Ir-ImNO	Fluorescent Dye	Cancer
Ir-ImNO is a mitochondria-targeted two-photon probe with excellent cell membrane permeability, capable of detecting both endogenous and exogenous cell membrane permeability. Ir-ImNO can monitor different immune response states of macrophages through various imaging techniques, including one-photon and two-photon phosphorescence imaging as well as phosphorescence lifetime imaging. Ir-ImNO can be utilized in the research to assist in the clinical monitoring of immunotherapy .

Cat. No.	Product Name	Type

HY-D2966

TMR-NO-BG	Fluorescent Dyes
TMR-NO-BG is a hybrid probe (Ex/Em: 557 nm/571 nm). TMR-NO-BG covalently connects the tetramethylrhodamine (TMR) lactone NO sensing unit with the SNAP-tag reactive benzyl guanine (BG) for achieving subcellular-specific nitric oxide (NO) detection .

HY-D3250

PYSNO	Fluorescent Dyes
PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λ_em=515-565 nm, λ_ex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases .

PYSNO

Fluorescent Dyes

PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λ_em=515-565 nm, λ_ex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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