Ir-ImNO
Ir-ImNO is a mitochondria-targeted two-photon probe with excellent cell membrane permeability, capable of detecting both endogenous and exogenous cell membrane permeability. Ir-ImNO can monitor different immune response states of macrophages through various imaging techniques, including one-photon and two-photon phosphorescence imaging as well as phosphorescence lifetime imaging. Ir-ImNO can be utilized in the research to assist in the clinical monitoring of immunotherapy.
For research use only. We do not sell to patients.
- Formula: C49H36ClIrN6
- Molecular Weight:936.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Ir-ImNO (5 μM; 6-48 h) exhibits low cytotoxicity in 4T1 and RAW 264.7 cells, maintaining a cell survival rate of over 80% after 48 hours of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 936.52
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Formula C49H36ClIrN6
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SMILES
NC1=C(NCC2=CC=CC=C2)C3=CC=C[N]4=C3C5=C1C=CC=[N]5[Ir+3]67([N]8=C9C(C=CC=C9)=CC=C8C%10=CC=CC=[C-]%107)4[N]%11=C%12C(C=CC=C%12)=CC=C%11C%13=CC=CC=[C-]%136.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ir-ImNO Related Classifications
HY-161476 Related Classifications
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