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Phosphofructokinase1

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標的別:	Phosphatase (3)
研究分野別:	Metabolic Disease (3)

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製品番号	製品名	Target	研究分野	構造式

HY-170404

KF-52	Phosphatase	Metabolic Disease
KF-52 is a phosphofructokinase-1 (PFK1) inhibitor with an IC₅₀ value of 2.1 μM. KF-52 significantly increases the ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR). KF-52 is applicable to research related to mitochondrial dysfunction ^[1].

HY-P2819

*Phosphofructokinase 1* PFK1	Phosphatase	Metabolic Disease
Phosphofructokinase 1 (PFK1) is a major regulatory enzyme targeting glycolytic pathway. Phosphofructokinase 1 catalyzes the phosphorylation of fructose-6-phosphate (F6P) to fructose-1,6-bisphosphate (F1,6BP) to regulate glycolytic flux. Phosphofructokinase 1 is promising for research of cancer metabolic reprogramming (e.g., hypoxia adaptation, oxidative stress resistance) and metabolic syndromes ^[1].

HY-170404A

KF-52 enantiomer	Phosphatase	Metabolic Disease
KF-52 enantiomer is an enantiomer of KF-52 (HY-170404). KF-52 is a phosphofructokinase-1 (PFK1) inhibitor ^[1].

Species:	Human (2)
Tag:	His (2) GST (1)
Source:	HEK293 (1) Sf9 insect cells (1)

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HY-P71090

	PFKM Protein, Human (HEK293, His) 6-Phosphofructokinase; muscle type; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase; PFKM; PFKX	Human	HEK293
	PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (HEK293, His) is the recombinant human-derived PFKM protein, expressed by HEK293 , with C-6*His labeled tag.

HY-P74635

	PFKM Protein, Human (sf9, His-GST) ATP-dependent 6-Phosphofructokinase; ATP-PFK; PFK-M; Phosphohexokinase	Human	Sf9 insect cells
	PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (sf9, His-GST) is the recombinant human-derived PFKM protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

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Host:	Mouse (1) Rabbit (2)
Reactivity:	Human (3) Rat (3) Mouse (3)
Application:	ICC/IF (3) IP (2) WB (3) ELISA (1)
Isotype:	IgG (2) IgG1 (1)
Conjugation:	Non-conjugated (3)
Clonality:	Monoclonal (2) Recombinant (1)
Modification:	Unmodified (2)

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HY-P85892

	PFKP Antibody (YA5584) PFKP; PFKF; 6-Phosphofructokinase type C; 6-Phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase	WB, ICC/IF, ELISA	Human, Mouse, Rat
	PFKP Antibody (YA5584) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PFKP.

HY-P81854

	PFKP Antibody (YA1599) PFKP; PFKF; 6-Phosphofructokinase type C; 6-Phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase	WB, ICC/IF, IP	Human, Mouse, Rat
	PFKP Antibody (YA1599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFKP.

HY-P81854A

	PFKP Antibody (YA1599)(PBS only) PFKP; PFKF; 6-Phosphofructokinase type C; 6-Phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase	WB, ICC/IF, IP	Human, Mouse, Rat
	PFKP Antibody (YA1599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFKP.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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