1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PFKM Protein, Human (sf9, His-GST)

PFKM Protein, Human (sf9, His-GST)

Cat. No.: HY-P74635
Handling Instructions

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (sf9, His-GST) is the recombinant human-derived PFKM protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PFKM Protein, Human (sf9, His-GST) is 779 a.a., with molecular weight of ~113 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (sf9, His-GST) is the recombinant human-derived PFKM protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PFKM Protein, Human (sf9, His-GST) is 779 a.a., with molecular weight of ~113 kDa.

Background

The PFKM protein assumes a pivotal role in cellular metabolism by catalyzing the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP as a substrate. This enzymatic activity marks the initial and committing step of glycolysis, a fundamental metabolic pathway central to energy production. By facilitating the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, PFKM plays a key role in regulating the entry of glucose into the glycolytic pathway, thereby influencing downstream energy production and cellular metabolic processes. The significance of PFKM in catalyzing this essential reaction underscores its importance in orchestrating the metabolic flux and maintaining cellular energy balance.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P08237 (T2-V780)

Gene ID
Molecular Construction
N-term
His-GST
PFKM (T2-V780)
Accession # P08237
C-term
Synonyms
ATP-dependent 6-phosphofructokinase; ATP-PFK; PFK-M; Phosphohexokinase
Molecular Weight

Approximately 113 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PFKM Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PFKM Protein, Human (sf9, His-GST)
Cat. No.:
HY-P74635
Quantity:
MCE Japan Authorized Agent: