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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PFKM Protein, Human (HEK293, His)

PFKM Protein, Human (HEK293, His)

Cat. No.: HY-P71090
COA Handling Instructions

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (HEK293, His) is the recombinant human-derived PFKM protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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50 μg $360 In-stock
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  • Biological Activity

  • Technical Parameters

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Description

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (HEK293, His) is the recombinant human-derived PFKM protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The PFKM protein assumes a pivotal role in cellular metabolism by catalyzing the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP as a substrate. This enzymatic activity marks the initial and committing step of glycolysis, a fundamental metabolic pathway central to energy production. By facilitating the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, PFKM plays a key role in regulating the entry of glucose into the glycolytic pathway, thereby influencing downstream energy production and cellular metabolic processes. The significance of PFKM in catalyzing this essential reaction underscores its importance in orchestrating the metabolic flux and maintaining cellular energy balance.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P08237-1 (T2-V780)

Gene ID
Molecular Construction
N-term
PFKM (T2-V780)
Accession # P08237-1
6*His
C-term
Synonyms
6-phosphofructokinase; muscle type; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase; PFKM; PFKX
AA Sequence

THEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Molecular Weight

Approximately 93.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 mM EDTA, 5% Trehalose, pH 6.9.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PFKM Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PFKM Protein, Human (HEK293, His)
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HY-P71090
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