2. Immunology/Inflammation
  3. Transmembrane Glycoprotein
  4. SYL3C aptamer sodium

SYL3C aptamer sodium is a DNA aptamer that targets epithelial cell adhesion molecule (EpCAM) on the surface of cancer cells. SYL3C aptamer sodium targets a variety of human cancer cell lines including MDA-MB-231, Kato III, HT-29, T47D and SW480. The Kd values of SYL3C aptamer sodium against breast cancer cell line MDA-MB-231 and gastric cancer cell line Kato III are 38 nM and 67 nM, respectively. SYL3C aptamer sodium possesses stability, high binding affinity and selectivity, and can be used for targeted cancer cell imaging and circulating tumor cell detection.

For research use only. We do not sell to patients.

DNA, 5'-CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG-3', sodium salt

SYL3C aptamer sodium Chemical Structure

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

SYL3C aptamer sodium Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SYL3C aptamer sodium is a DNA aptamer that targets epithelial cell adhesion molecule (EpCAM) on the surface of cancer cells. SYL3C aptamer sodium targets a variety of human cancer cell lines including MDA-MB-231, Kato III, HT-29, T47D and SW480. The Kd values of SYL3C aptamer sodium against breast cancer cell line MDA-MB-231 and gastric cancer cell line Kato III are 38 nM and 67 nM, respectively. SYL3C aptamer sodium possesses stability, high binding affinity and selectivity, and can be used for targeted cancer cell imaging and circulating tumor cell detection[1].

IC50 & Target

Epithelial cell adhesion molecule (EpCAM)[1]
In Vitro

SYL3C aptamer (various concentrations; 30 min at 37 °C) binds to human breast cancer MDA-MB-231 cells with a Kd of 38 nM and to human gastric cancer Kato III cells with a Kd of 67 nM, exhibiting stronger affinity than the full-length SYL3 aptamer[1].
SYL3C aptamer (200 nM; 30 min flow cytometry, 40 min confocal imaging) specifically binds to human EpCAM-positive cancer cell lines (MDA-MB-231, Kato III, HT-29, T47D, SW480) and does not bind to human EpCAM-negative cell lines (HEK-293T, U118MG, Ramos)[1].
SYL3C aptamer (200 nM; 40 min at 4 °C) selectively labels EpCAM-positive human breast cancer MDA-MB-231 cells and human gastric cancer Kato III cells for fluorescence imaging, with no detectable labeling of EpCAM-negative human embryonic kidney HEK-293T cells[1].
SYL3C aptamer (30 min cell incubation) immobilized on a solid surface captures human gastric cancer Kato III cells with up to 63% efficiency and 80% purity, with minimal non-specific capture of EpCAM-negative Ramos cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SYL3C aptamer sodium Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: HT-29, HEK293, CHO cells
Concentration: 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1 μM
Incubation Time: 24 h
Result: Maintained cell viability above 80% across all tested concentrations and cell lines after 24-hour incubation.
In Vivo

Cy7-SYL3C (1 nM; i.v.; single tail vein injection) enables targeted near-infrared fluorescence imaging of EpCAM-positive colorectal cancer xenografts via renal metabolism, and conjugation with DNA tetrahedra (forming Cy7-DTN-SYL3C (1 nM; i.v.; single tail vein injection)) prolongs circulation time, doubles tumor uptake over 24 hours, and increases the tumor-to-background ratio to 3.05 while maintaining specific EpCAM binding[2].
DTN-SYL3C (0.02 mg/kg; i.v.; single tail vein injection) is biocompatible in healthy BALB/c mice, causing no observable damage to major organs after 24 hours[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c Nude (female, 5-6 weeks old, subcutaneous colorectal cancer xenograft model)[2]
Dosage: 1 nM (Cy7-SYL3C); 1 nM (Cy7-DTN-SYL3C)
Administration: i.v.; single tail vein injection
Result: Exhibited primary renal metabolism, with fluorescence signals localized in the liver and kidneys for the first 4 hours, then concentrated in the kidneys with decreasing intensity over time.
Showed enhanced, prolonged fluorescence signals at the tumor site, confirming specific binding to EpCAM.
Had kidney fluorescence intensity 1.8 to 2 times higher than Cy7-SYL3C from 2 to 24 hours (statistically significant), indicating prolonged circulation time.
Had tumor fluorescence intensity twice as high as Cy7-SYL3C over 24 hours.
Displayed a dispersed dot-like binding pattern in tumor tissue with a Pearson’s coefficient of 0.32 against EpCAM (Cy5-SYL3C).
Animal Model: BALB/c (6-7 weeks old, healthy)[2]
Dosage: 0.02 mg/kg (equivalent to 1 nM)
Administration: i.v.; single tail vein injection
Result: Showed no significant differences in H&E staining of major organs compared to the PBS control group, with no evident tissue damage observed.
Molecular Weight

14838.5 (free acid)

Appearance

Solid

Color

White to off-white

SMILES

[SYL3C aptamer (sodium)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

SYL3C aptamer sodium Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SYL3C aptamerTransmembrane GlycoproteinDNA TetrahedraEpCAM-negative cellsxenograft micecolorectal cancerEpCAMhuman recombinant EpCAM proteinbreast cancergastric cancerMDA-MB-231 cellslive cancer cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
SYL3C aptamer sodium
Cat. No.:
HY-160066
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.