2. Stem Cell/Wnt TGF-beta/Smad
  3. TGF-beta/Smad
  4. TGFβ1-IN-4

TGFβ1-IN-4 is a TGF-β1 inhibitor. TGFβ1-IN-4 inhibits myofibroblast activation and epithelial-mesenchymal transition in vitro. TGFβ1-IN-4 alleviates renal fibrosis in a mouse model of renal fibrosis. TGFβ1-IN-4 can be used for research on fibrotic diseases such as renal fibrosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

TGFβ1-IN-4

TGFβ1-IN-4 Chemical Structure

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TGFβ1-IN-4 Related Antibodies

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Description

TGFβ1-IN-4 is a TGF-β1 inhibitor. TGFβ1-IN-4 inhibits myofibroblast activation and epithelial-mesenchymal transition in vitro. TGFβ1-IN-4 alleviates renal fibrosis in a mouse model of renal fibrosis. TGFβ1-IN-4 can be used for research on fibrotic diseases such as renal fibrosis[1].

In Vitro

TGFβ1-IN-4 (Peptide 27) (1-10 μM, 24-48 h) inhibits myofibroblast activation, fibrosis progression and epithelial-mesenchymal transition (EMT) in TGF-β1-induced NIH-3T3 and HK-2 cells[1].
TGFβ1-IN-4 (0-72 h) exhibits strong enzymatic hydrolysis resistance in mouse serum[1].
TGFβ1-IN-4 (1 mM) binds to albumin, as determined by isothermal titration calorimetry[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TGFβ1-IN-4 Related Antibodies

Western Blot Analysis[1]

Cell Line: NIH-3T3 cells treated with TGF-β1
Concentration: 1, 2, 5, 10 μM
Incubation Time: 48 h
Result: Reduced the expression of
fibronectin and α-SMA in a dose-dependent manner.
Suppressed the expression of collagen I, MMP2, and vimentin.

Real Time qPCR[1]

Cell Line: NIH-3T3 cells treated with TGF-β1
Concentration: 1, 2 μM
Incubation Time: 48 h
Result: Decreased expression levels of fibrosis-related genes, including fibronectin, collagen I, MMP2, vimentin, α-SMA, and TGF-β1.

Western Blot Analysis[1]

Cell Line: HK-2 cells treated with TGF-β1
Concentration: 1, 2, 5, 10 μM
Incubation Time: 24 h
Result: Significantly inhibited the expression of fibronectin and α-SMA.
Reduced the expression of collagen I, MMP2, and vimentin while increasing the expression of E-cadherin.

Real Time qPCR[1]

Cell Line: HK-2 cells treated with TGF-β1
Concentration: 1, 5 μM
Incubation Time: 24 h
Result: Demonstrated consistent improvement effects on
the mRNA levels of fibrosis-related genes.
Parmacokinetics
Species Dose Route T1/2 AUC0-t AUC0-∞ CL Vd MRT
Mice[1] 2.8 mg/kg i.v. 1.077 h 1.894 μg·h/mL 1.938 μg·h/mL 0.028 L/h 0.044 L 0.337 h
In Vivo

Peptide 27 (0.05-1.25 mg/kg; i.p.; daily; 7 days) effectively alleviates unilateral ureteral obstruction-induced renal fibrosis in a mouse model of renal fibrosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice (male, 20-25 g)[1]
Dosage: 0.05, 0.25, 1.25 mg/kg
Administration: i.p.; daily; 7 days
Result: Improved kidney condition.
Prevented the formation of kidney inflammation and fibrosis. Dose-dependently reduced ECM deposition and the expression of fibrosis marker mRNA.
Mitigated the progression of epithelial-mesenchymal transition (EMT).
Reduced UUO-induced renal fibrosis in mice within a dose range of 0.05 to 1.25 mg/kg.
Molecular Weight

1150.41

Formula

C51H83N13O13S2
Sequence

C12-{d-Asp}-His-Asn-Cys-Pro-Gln-{d-Ala}-Cys-NH2 (Hexanedithiol linker:Cys4-Cys8)
Sequence Shortening

C12-{d-Asp}-HNCPQ-{d-Ala}-C-NH2 (Hexanedithiol linker:Cys4-Cys8)
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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TGFβ1-IN-4 Related Classifications

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  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TGFβ1-IN-4TGF-beta/SmadTransforming growth factor betaTGF-β1NIH-3T3 cellsHK-2 cellsRenal fibrosis mouse modelRenal fibrosisInhibitorinhibitorinhibit

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