BChE-IN-50

BChE-IN-50 (compound W3) potently and selectively inhibits human butyrylcholinesterase with an IC₅₀ of 0.28 μM and a selectivity index > 357 over human acetylcholinesterase^[1].
BChE-IN-50 binds to the substrate-binding pocket of human butyrylcholinesterase with a total binding free energy of -54.38 kcal/mol, but does not occupy the acyl-binding pocket of the enzyme^[1].
BChE-IN-50 acts as a mixed-type human butyrylcholinesterase inhibitor with a K_i of 0.78 μM, an α value of 3.1, and a K_i' of 2.4 μM^[1].
BChE-IN-50 (1-100 μM; 24 h) exhibits low in vitro cytotoxicity to HT22, BV2, and SH-SY5Y cells^[1].
BChE-IN-50 (1-10 μM; 2 h pre-incubation, 24 h H₂O₂ exposure) protects BV2 and SH-SY5Y cells from H₂O₂-induced oxidative damage in a concentration-dependent manner^[1].
BChE-IN-50 (1-10 μM; 2 h pre-incubation, 24 h Aβ_1-42 exposure) protects SH-SY5Y cells from Aβ_1-42-induced damage in a concentration-dependent manner^[1].
BChE-IN-50 (1-5 μM; 2 h pre-incubation, 24 h LPS/Aβ_1-42 exposure) significantly reduces LPS-induced ROS production in BV2 cells and Aβ_1-42-induced ROS production in SH-SY5Y cells at concentrations of 1 and 5 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.