VT00065 

VT00065 is a covalent DHS inhibitor. VT00065 forms a redox-dependent covalent bond with the catalytic lysine K329 of DHS, locking the enzyme in an inactive state^[1].

VT00065 (100 mM; 24 h) covalently and specifically modifies the catalytic K329 residue of purified human DHS, suggesting it acts as a mechanism-based binder with potential to irreversibly inactivate the enzyme, though it exhibits low binding occupancy^[1].
VT00065 (2-20 mM; 9 min) exhibits weak, dose-dependent inhibition of deoxyhypusination activity in purified human DHS-eIF5A reactions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

128.20

C₅H₈N₂S

144163-68-8

Liquid (Density: 1.126±0.06 g/cm³)

Colorless to light yellow

CNCC1=NC=CS1

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Wilk P, et al. Crystallographic fragment screening supports tool compound discovery and reveals conformational flexibility in human deoxyhypusine synthase. Commun Chem. 2026;9(1):66. Published 2026 Jan 17.  [Content Brief]