2α-Ferrocenylmethyl-DHT
2α-Ferrocenylmethyl-DHT is a dihydrotestosterone-derived ferrocene-steroid conjugate. 2α-Ferrocenylmethyl-DHT inhibits the growth of various cancer cells. 2α-Ferrocenylmethyl-DHT induces S-phase cell cycle arrest in ovarian cancer cells, elevates intracellular iron levels, triggers ROS-dependent cell death, and disrupts the integrity of multicellular tumor spheroids of ovarian cancer cells. 2α-Ferrocenylmethyl-DHT can be used in the research of prostate cancer and ovarian cancer.
For research use only. We do not sell to patients.
- Formula: C30H40FeO
- Molecular Weight:472.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
2α-Ferrocenylmethyl-DHT (Compound 2) (3.12-50 μM; 72 h) potently inhibits the growth of OVCAR-3 (IC50 = 2.8 μM), PC-3 (IC50 = 16.8 μM), LNCaP (IC50 = 16 μM) cancer cells and non-malignant MRC-5 fibroblasts (IC50 = 8.5 μM), and exhibits moderate tumor selectivity toward OVCAR-3 cells[1].
2α-Ferrocenylmethyl-DHT (2.8 μM; 48 h, 72 h) induces time-dependent S-phase arrest in OVCAR-3 cells, with extremely low accumulation of Sub-G1 phase cells[1].
Treatment with 2α-Ferrocenylmethyl-DHT (50 μM; 24 h) increases intracellular iron levels in OVCAR-3 cells by 21-fold[1].
2α-Ferrocenylmethyl-DHT (0.3-30 μM; 72 h) induces ROS-dependent, caspase-independent cell death in OVCAR-3 cells[1].
2α-Ferrocenylmethyl-DHT (3.12-50 μM; 72 h) disrupts the integrity and inhibits the growth of OVCAR-3 multicellular tumor spheroids, with an IC50 of 14 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human prostate adenocarcinoma PC-3 cells, LNCaP cells, ovarian adenocarcinoma OVCAR-3 cells, non-malignant MRC-5 fetal lung fibroblasts
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Concentration:3.12, 6.25, 12.5, 25, 50 μM
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Incubation Time:72 h
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Result:Inhibited PC-3 cell growth with an IC50 of 16.8 μM.
Inhibited LNCaP cell growth with an IC50 of 16 μM.
Inhibited OVCAR-3 cell growth with an IC50 of 2.8 μM.
Inhibited MRC-5 cell growth with an IC50 of 8.5 μM.
Achieved a selectivity index of 3.03 for OVCAR-3 cells relative to MRC-5 cells.
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Cell Line:human ovarian adenocarcinoma OVCAR-3 cells
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Concentration:2.8 μM
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Incubation Time:48 h; 72 h
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Result:Increased S-phase population to 24.1=% after 72 h, compared to 10=% in untreated controls.
Reduced G1 phase population and elevated G2/M phase population after 72 h.
Increased Sub-G1 fraction to 9.3% after 72 h.
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Cell Line:3D multicellular tumor spheroids (MCTSs) of human ovarian adenocarcinoma OVCAR-3 cells
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Concentration:3.12, 6.25, 12.5, 25, 50 μM
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Incubation Time:72 h
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Result:Inhibited OVCAR-3 MCTS growth with an IC50 of 14 μM.
Induced weakening of intercellular contacts and loss of spheroid compactness starting at 6.25 μM.
Caused complete loss of spheroid architecture at 50 μM.
Chemical Information
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Molecular Weight 472.48
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Formula C30H40FeO
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SMILES
C[C@]1(C/2)[C@](CC[C@]3([H])[C@]1([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4O)([H])CCC2=C\c5cccc5.[][Fe][].c6cccc6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2α-Ferrocenylmethyl-DHT Related Classifications
HY-181688 Related Classifications
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