21-Hydroxyisoohchininolide
21-Hydroxyisoohchininolide is a salannin-type limonoid. 21-Hydroxyisoohchininolide inhibits lipopolysaccharide (LPS, HY-D1056)-induced nitric oxide production in mouse macrophages. 21-Hydroxyisoohchininolide exhibits cytotoxic activity against leukemia cells and breast cancer cells. 21-Hydroxyisoohchininolide suppresses phorbol 12-myristate 13-acetate (PMA) (HY-18739)-induced activation of Epstein-Barr virus early antigen in lymphocytes. 21-Hydroxyisoohchininolide can be used for the research of leukemia, breast cancer and inflammatory diseases.
For research use only. We do not sell to patients.
- CAS No.: 1429747-14-7
- Formula: C36H42O10
- Molecular Weight:634.71
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
21-Hydroxyisoohchininolide (1×10-6-1×10-4 M; 48 h) exhibits cytotoxicity against HL60 human leukemia cells (IC50 = 22.7 ± 1.6 μM) and SK-BR-3 human breast cancer cells (IC50 = 91.5 ± 2.2 μM), but shows no cytotoxicity against A549 human lung cancer cells and AZ521 human stomach cancer cells after 48 h of treatment[1].
21-Hydroxyisoohchininolide (1×10-6-1×10-4 M; 2 h pre-incubation, 16 h LPS co-incubation) inhibits LPS-induced nitric oxide production in RAW 264.7 murine macrophage cells with an IC50 of 87.3 ± 4.5 μM, with no cytotoxicity to the cells after 16 h of LPS co-incubation[1].
21-Hydroxyisoohchininolide (10-1000 molar ratio relative to 32 pmol TPA) inhibits TPA-induced EBV-EA activation in Raji cells with an IC50 of 493 molar ratio relative to 32 pmol TPA, with 60% cell viability at the highest tested molar ratio of 1000:1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HL60 human leukemia cells, AZ521 human stomach cancer cells, A549 human lung cancer cells, SK-BR-3 human breast cancer cells
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Concentration:1, 10 and 100 μM
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Incubation Time:48 h
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Result:Exhibited cytotoxicity against HL60 cells with an IC50 of 22.7 μM.
Exhibited cytotoxicity against SK-BR-3 cells with an IC50 of 91.5 μM.
Showed no cytotoxicity (IC50 > 100 μM) against A549 and AZ521 cells.
Chemical Information
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CAS No. 1429747-14-7
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Molecular Weight 634.71
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Formula C36H42O10
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SMILES
C[C@]12[C@@]3([H])[C@](OC[C@@]3([C@@H](C[C@@H]2OC(/C=C/C4=CC=CC=C4)=O)O)C)([H])[C@]5([H])[C@]([C@@H]1CC(OC)=O)(C6=C(C)[C@H](C7=CC(OC7O)=O)C[C@@]6([H])O5)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
21-Hydroxyisoohchininolide Related Classifications
HY-N18117 Related Classifications
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