2. Immunology/Inflammation
  3. Aryl Hydrocarbon Receptor
  4. CH-223191

CH-223191 

Cat. No.: HY-12684 Purity: 98.71%
COA Handling Instructions

CH-223191 is a potent and specific antagonist of aryl hydrocarbon receptor (AhR). CH-223191 inhibits TCDD-mediated nuclear translocation and DNA binding of AhR, and inhibits TCDD-induced luciferase activity with an IC50 of 0.03 μM.

For research use only. We do not sell to patients.

CH-223191 Chemical Structure

CH-223191 Chemical Structure

CAS No. : 301326-22-7

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Customer Review

Based on 35 publication(s) in Google Scholar

Top Publications Citing Use of Products

29 Publications Citing Use of MCE CH-223191

WB
RT-PCR
IF
Proliferation Assay

    CH-223191 purchased from MCE. Usage Cited in: Nat Metab. 2023 Feb 13.  [Abstract]

    CH223191 suppresses GSK3β and Akt phosphorylation in chow-fed mice.

    CH-223191 purchased from MCE. Usage Cited in: Chemosphere. 2018 Oct 23;216:372-378.  [Abstract]

    AhR mediates EOM-inhibited cardiac differentiation by downregulating β-catenin expression in P19 cells. Percentage of cTnT positive cells at differentiation day 14. The concentrations of CH (CH223191, AhR antagonist) and CHIR (CHIR99021, Wnt activator) are 0.25 and 0.5 μM respectively.

    CH-223191 purchased from MCE. Usage Cited in: Chemosphere. 2018 Oct 23;216:372-378.  [Abstract]

    Proliferation of P19 cells exposed to EOM in the presence or absence of CH. Represent images of EdU staining (red) and the quantification results.

    CH-223191 purchased from MCE. Usage Cited in: Chemosphere. 2018 Oct 23;216:372-378.  [Abstract]

    AhR mediates EOM-inhibited cardiac differentiation by downregulating β-catenin expression in P19 cells. Percentage of cTnT positive cells at differentiation day 14. The concentrations of CH (CH223191, AhR antagonist) and CHIR (CHIR99021, Wnt activator) are 0.25 and 0.5 μM respectively.

    CH-223191 purchased from MCE. Usage Cited in: Chemosphere. 2018 Oct 23;216:372-378.  [Abstract]

    AhR mediates EOM-inhibited cardiac differentiation by downregulating β-catenin expression in P19 cells. mRNA expression changes of β-catenin by Q-PCR. The concentrations of CH (CH223191, AhR antagonist) and CHIR (CHIR99021, Wnt activator) are 0.25 and 0.5 μM respectively.

    CH-223191 purchased from MCE. Usage Cited in: Toxicology. 2016 May 20;355-356:31-38.  [Abstract]

    Western analysis of β-catenin protein level in zebrafish embryos exposed to EOM in the presence or absence of CH/CHIR (n = 3). Different letters indicate significant differences. Moreover, both CH and CHIR attenuate the EOM-induced changes in the protein level of b-catenin and in EROD activity.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    CH-223191 is a potent and specific antagonist of aryl hydrocarbon receptor (AhR). CH-223191 inhibits TCDD-mediated nuclear translocation and DNA binding of AhR, and inhibits TCDD-induced luciferase activity with an IC50 of 0.03 μM[1].

    In Vitro

    CH-223191 (0.1-10 μM; pre-treated 1 hour) inhibits TCDD-caused cytochrome P450 1A1 mRNA expression in a in dose-dependent manner[1].
    CH-223191 (0.1-10 μM; pre-treated 1 hour) causes a concentration-dependent inhibition of TCDD-induced cytochrome P450 enzyme activity[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    RT-PCR[1]

    Cell Line: HepG2 cells
    Concentration: 0.1-10 μM
    Incubation Time: 1 hour
    Result: Caused inhibition of TCDD-induced cytochrome P450 mRNA expression.

    Western Blot Analysis[1]

    Cell Line: HepG2 cells
    Concentration: 0.1-10 μM
    Incubation Time: 1 hour
    Result: Decreased TCDD-caused cytochrome P450 1A1 protein Treatment.
    In Vivo

    CH-223191 (10 mg/kg; once a day; 25 days) suppresses cytochrome P450 1A1 expression and the intrahepatocyte fat content in liver, reduces activity of AST and ALT in TCDD-treated mice[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male ICR mice (6 weeks old)[1]
    Dosage: 10 mg/kg
    Administration: 10 mg/kg; once a day; 25 days
    Result: Prevented TCDD-elicited cytochrome P450 induction, liver toxicity, and wasting syndrome in mice.
    Molecular Weight

    333.39

    Appearance

    Solid

    Formula

    C19H19N5O
    CAS No.
    SMILES

    O=C(C1=CC=NN1C)NC2=CC=C(/N=N/C3=CC=CC=C3C)C=C2C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 62.5 mg/mL (187.47 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9995 mL 14.9974 mL 29.9949 mL
    5 mM 0.5999 mL 2.9995 mL 5.9990 mL
    10 mM 0.2999 mL 1.4997 mL 2.9995 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.60%

    COA (247 KB) LCMS (111 KB)

    Handling Instructions (2659 KB)
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    CH-223191301326-22-7CH223191CH 223191Aryl Hydrocarbon ReceptorAhRInhibitorinhibitorinhibit

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