1. Peptides

Myelin Basic Protein (MBP) (68-82), guinea pig 

Cat. No.: HY-P1048
Handling Instructions

Myelin Basic Protein (MBP) (68-82), guinea pig is a fragment of myelin basic protein (MBP).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Myelin Basic Protein (MBP) (68-82), guinea pig Chemical Structure

Myelin Basic Protein (MBP) (68-82), guinea pig Chemical Structure

CAS No. : 98474-59-0

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1 mg USD 90 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
10 mg USD 280 In-stock
Estimated Time of Arrival: December 31
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Myelin Basic Protein (MBP) (68-82), guinea pig is a fragment of myelin basic protein (MBP).

In Vitro

Multiple sclerosis is the most common autoimmune disorder affecting the central nervous system. In this study, whole blood samples are analyzed for activation capacity and the activatability of CD4+ and CD8+ T-lymphocytes by human total myelin basic protein (MBP), human MBP 104-118 fragment, and guinea pig MBP (68-82) fragment. A significant increase in the number of activated T-lymphocytes was observed in the whole blood. For all three tested MBPs, this increase in activated CD4+ and CD8+ T-lymphocytes is statistically significant (p<0.01). However, this increase in activated T-cells is most prominent following incubation with human total MBP, followed by human 104-118 fragment; the smallest increase is observed following incubation with guinea pig MBP (68-82) fragment (human total MBP>huMBP-104-118>guinea pig MBP (68-82))[1].

In Vivo

Whether pretreatment with bee venom acupuncture (BVA) from the same day of MBP (68-82) immunization can affect the induction and progression of experimental autoimmune encephalomyelitis (EAE) and weight loss is examined. At 5-9 days after immunization, rats in the myelin basic protein (MBP) group start displaying partial loss of tail tonus (clinical signs, 0.5) in a freely moving environment. At 10-16 days after immunization, most of the rats in the MBP group display more severe symptoms of neurological deficit including paraparesis of the hindlimb, paraplegia, tetraparesis, and tetraplegia. In contrast, rats in the MBP + BVA group display relatively slight neurological deficits in a dose-dependent manner at 11-15 days after immunization, compared to the rats in the MBP group. The onset of symptoms is slightly delayed (BVA 0.8 mg/kg, 6.4±0.6 days) and the maximal clinical score is markedly decreased (BVA 0.25 mg/kg, 3.7±0.2; BVA 0.8 mg/kg, 2.8±0.3), compared to that in the MBP group. At this time, the mean body weight of rats in the MBP group is decreased as compared to that of rats in the normal group, but it is significantly increased in rats of the MBP + BVA group as compared to rats in the MBP group[2].

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
Kinase Assay
[1]

For each individual, the whole blood sample is typically divided into six 1 mL aliquots/tubes. Concanavalin A is added to tube 1 (positive control). Tube 2 is left untreated. Tube 3 is treated with human albumin as a negative control. Human total MBP, human MBP 104-118 fragment and guinea pig MBP (68-82) are added to tubes 4, 5 and 6, respectively. All proteins are added to a final concentration of 2 µg/mL. All experiments (incubations and flow cytometric analysis) are performed in duplicate for each subject[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Rats[2]
Ten-week-old female Lewis rats are divided into the following two experimental groups: BVA-pretreated (every 3 days from 20 min before immunization) and BVA-posttreated (daily from days 10-15 after immunization) groups. Each experimental group is subdivided into the following five groups: normal [saline, subcutaneous (s.c.)+saline, s.c.], MBP [250 μg of myelin basic protein MBP (68-82), s.c.+saline, s.c., ST36 acupoint], MBP+BVA 0.25 [250 μg of MBP (68-82), s.c.+0.25 mg/kg body weight of BV, s.c., ST36 acupoint], MBP+BVA 0.8 [250 μg of MBP (68-82), s.c.+0.8 mg/kg body weight of BV, s.c., ST36 acupoint], and BVA alone [saline, s.c.+0.8 mg/kg body weight of BV, s.c., ST36 acupoint] groups. EAE is induced with an emulsion containing 250 μg of MBP (68-82) and 100 μg of Mycobacterium M. tuberculosis per mL of incomplete Freund’s adjuvant. A total of 0.2 mL of this emulsion is injected s.c. into the two hind footpads of rats except for those in the normal group and BVA alone group. Additionally, rats receive intraperitoneal (i.p.) injections of 200 ng of pertussis toxin on days 0 and 2. Rats in the normal group are treated with saline alone instead of MBP (68-82) peptide, pertussis toxin, or BVA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1736.79

Formula

C₇₁H₁₁₃N₂₃O₂₈

CAS No.

98474-59-0

Sequence

Tyr-Gly-Ser-Leu-Pro-Gln-Lys-Ser-Gln-Arg-Ser-Gln-Asp-Glu-Asn

Sequence Shortening

YGSLPQKSQRSQDEN

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Myelin Basic Protein (MBP) (68-82), guinea pig
Cat. No.:
HY-P1048
Quantity:

Myelin Basic Protein (MBP) (68-82), guinea pig

Cat. No.: HY-P1048