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NAI-N3 

Cat. No.: HY-103006 Purity: 98.05%
Handling Instructions

NAI-N3 is a novel RNA acylation reagent that enables RNA purification.

For research use only. We do not sell to patients.

NAI-N3 Chemical Structure

NAI-N3 Chemical Structure

CAS No. : 1612756-29-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
5 mg USD 84 In-stock
Estimated Time of Arrival: December 31
10 mg USD 132 In-stock
Estimated Time of Arrival: December 31
25 mg USD 276 In-stock
Estimated Time of Arrival: December 31
50 mg USD 468 In-stock
Estimated Time of Arrival: December 31
100 mg USD 828 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

NAI-N3 is a novel RNA acylation reagent that enables RNA purification.

In Vitro

The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50–100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3–treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3– and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1].

Molecular Weight

228.21

Formula

C₁₀H₈N₆O

CAS No.

1612756-29-2

SMILES

O=C(C1=CC=CN=C1CN=[N+]=[N-])N2C=CN=C2

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 115 mg/mL (503.92 mM)

H2O : 1 mg/mL (4.38 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3819 mL 21.9096 mL 43.8193 mL
5 mM 0.8764 mL 4.3819 mL 8.7639 mL
10 mM 0.4382 mL 2.1910 mL 4.3819 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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NAI-N3
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HY-103006
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