N-Methylisatoic anhydride
Based on 1 Customer Validation
N-Methylisatoic anhydride is a heteronucleoside anhydride derivative conjugated with biotin and a disulfide linker for specific 2'-OH acylation of RNA. Biotin-conjugated N-Methylisatoic anhydride can selectively capture RNA from a mixture of biological RNA and DNA.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 10328-92-4
- Formula: C9H7NO3
- Molecular Weight:177.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
N-Methylisatoic anhydride is a 20-OH selective acylation agents of RNAs, and widely used for resolving secondary RNA structures using the SHAPE (Selective 20-Hydroxyl Acylation Analyzed by Primer Extension) technology[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10328-92-4
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Appearance Solid
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Molecular Weight 177.16
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Formula C9H7NO3
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Color White to yellow
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SMILES
O=C(OC1=O)N(C)C2=C1C=CC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (282.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6446 mL | 28.2232 mL | 56.4465 mL | 141.1162 mL |
| 5 mM | 1.1289 mL | 5.6446 mL | 11.2893 mL | 28.2232 mL | |
| 10 mM | 0.5645 mL | 2.8223 mL | 5.6446 mL | 14.1116 mL | |
| 15 mM | 0.3763 mL | 1.8815 mL | 3.7631 mL | 9.4077 mL | |
| 20 mM | 0.2822 mL | 1.4112 mL | 2.8223 mL | 7.0558 mL | |
| 25 mM | 0.2258 mL | 1.1289 mL | 2.2579 mL | 5.6446 mL | |
| 30 mM | 0.1882 mL | 0.9408 mL | 1.8815 mL | 4.7039 mL | |
| 40 mM | 0.1411 mL | 0.7056 mL | 1.4112 mL | 3.5279 mL | |
| 50 mM | 0.1129 mL | 0.5645 mL | 1.1289 mL | 2.8223 mL | |
| 60 mM | 0.0941 mL | 0.4704 mL | 0.9408 mL | 2.3519 mL | |
| 80 mM | 0.0706 mL | 0.3528 mL | 0.7056 mL | 1.7640 mL | |
| 100 mM | 0.0564 mL | 0.2822 mL | 0.5645 mL | 1.4112 mL |
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